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- W2137121488 endingPage "6216" @default.
- W2137121488 startingPage "6204" @default.
- W2137121488 abstract "ABSTRACT Yersinia type III machines secrete protein substrates across the bacterial envelope and, following assembly of their secretion needles, transport effector Yops into host cells. According to their destination during type III secretion, early, middle, and late secretion substrates can be distinguished; however, the signals and mechanisms whereby these proteins are recognized and transported by the secretion machine are not understood. Here, we examine several hybrids between secretion substrates and the impassable reporter protein glutathione S -transferase (GST). YscP-GST and YopR-GST blocked type III secretion; however, YscF-, YopD-, YopN-, and LcrV-GST did not. Unlike YopR-GST, which can block type III machines only during their assembly, expression of YscP-GST led to an immediate and complete block of all secretion. The secretion signal of YscP was mapped to its first 10 codons or amino acids; however, YscP Δ2-15 -GST, lacking this secretion signal, imposed a partial blockade. YscP-GST copurified with the type III ATPase complex (YscN, YscL, and YscQ) and with YscO, suggesting that the association of specific machine components with the impassable substrate may cause the block in type III secretion." @default.
- W2137121488 created "2016-06-24" @default.
- W2137121488 creator A5001395912 @default.
- W2137121488 creator A5004086899 @default.
- W2137121488 creator A5064843238 @default.
- W2137121488 creator A5083683183 @default.
- W2137121488 date "2008-09-15" @default.
- W2137121488 modified "2023-09-27" @default.
- W2137121488 title "Impassable YscP Substrates and Their Impact on the <i>Yersinia enterocolitica</i> Type III Secretion Pathway" @default.
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- W2137121488 doi "https://doi.org/10.1128/jb.00467-08" @default.
- W2137121488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2546781" @default.