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• W2137158805 abstract "One of the emerging biopolymers that are currently under active investigation is bacterial poly(γ-glutamic acid) (γ-PGA). However, before its full industrial exploitation, a substantial increase in microbial productivity is required. γ-PGA obtained from the Bacillus subtilis laboratory strain 168 offers the advantage of a producer characterized by a well defined genetic framework and simple manipulation techniques. In this strain, the knockout of genes for the major γ-PGA degrading enzymes, pgdS and ggt, leads to a considerable improvement in polymer yield, which attains levels analogous to the top wild γ-PGA producer strains. This study highlights the convenience of using the laboratory strain of B. subtilis over wild isolates in designing strain improvement strategies aimed at increasing γ-PGA productivity. Biotechnol. Bioeng. 2013; 110: 2006–2012. © 2013 Wiley Periodicals, Inc." @default.
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• W2137158805 date "2013-02-15" @default.
• W2137158805 modified "2023-09-25" @default.
• W2137158805 title "Knockout of<i>pgdS</i>and<i>ggt</i>genes improves γ-PGA yield in<i>B. subtilis</i>" @default.
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• W2137158805 doi "https://doi.org/10.1002/bit.24846" @default.
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