Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137231640> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2137231640 abstract "RNA quality and quantity are important factors for ensuring the accuracy of gene expression analysis and other RNA-based downstream applications. Extraction of high quality nucleic acids is difficult from neuronal cells and brain tissues as they are particularly rich in lipids. In addition, most common RNA extraction methods are phenol-based, resulting in RNA that may be incompatible with downstream applications such as gene expression.In this work, a comparative analysis of the RNA quality obtained from SK-N-MC cells was performed using six commonly used RNA isolation kits: two phenol-based kits and four non-phenol based kits. The non-phenol based kits tested AxyPrep Multisource Total RNA Miniprep, RNeasy Mini, EasySpin and Ilustra RNAspin Mini RNA Isolation, all performed well and resulted in the isolation of high quality RNA, as evaluated by A260/A280. The RNA extracted with AxyPrep Multisource Total RNA Miniprep, RNeasy Mini and EasySpin provided the highest RNA yields. In particular, the RNA isolated by AxyPrep Multisource Total RNA Miniprep Kit did not show any detectable genomic DNA contamination even without previous DNase treatment or after RNA direct PCR amplification using universal 18S primers.The RNA extracted from SK-N-MC cells with AxyPrep Multisource Total RNA Miniprep Kit was superior with respect to the RNA quality and concentration. This kit does not use aggressive organic solvents and RNA free of genomic DNA was isolated without the need for DNase treatment." @default.
- W2137231640 created "2016-06-24" @default.
- W2137231640 creator A5014022727 @default.
- W2137231640 creator A5014441247 @default.
- W2137231640 creator A5038256279 @default.
- W2137231640 creator A5060451599 @default.
- W2137231640 date "2011-01-06" @default.
- W2137231640 modified "2023-10-06" @default.
- W2137231640 title "Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma" @default.
- W2137231640 cites W12153005 @default.
- W2137231640 cites W1971377917 @default.
- W2137231640 cites W1978979520 @default.
- W2137231640 cites W2001530093 @default.
- W2137231640 cites W2074761621 @default.
- W2137231640 cites W2090641374 @default.
- W2137231640 cites W2096976625 @default.
- W2137231640 cites W2102430660 @default.
- W2137231640 cites W2107673238 @default.
- W2137231640 cites W2118537438 @default.
- W2137231640 cites W2136935640 @default.
- W2137231640 cites W2147734237 @default.
- W2137231640 cites W2157017203 @default.
- W2137231640 doi "https://doi.org/10.1186/1756-0500-4-3" @default.
- W2137231640 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3050700" @default.
- W2137231640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21211020" @default.
- W2137231640 hasPublicationYear "2011" @default.
- W2137231640 type Work @default.
- W2137231640 sameAs 2137231640 @default.
- W2137231640 citedByCount "42" @default.
- W2137231640 countsByYear W21372316402012 @default.
- W2137231640 countsByYear W21372316402013 @default.
- W2137231640 countsByYear W21372316402014 @default.
- W2137231640 countsByYear W21372316402015 @default.
- W2137231640 countsByYear W21372316402016 @default.
- W2137231640 countsByYear W21372316402017 @default.
- W2137231640 countsByYear W21372316402018 @default.
- W2137231640 countsByYear W21372316402019 @default.
- W2137231640 countsByYear W21372316402020 @default.
- W2137231640 countsByYear W21372316402021 @default.
- W2137231640 countsByYear W21372316402022 @default.
- W2137231640 countsByYear W21372316402023 @default.
- W2137231640 crossrefType "journal-article" @default.
- W2137231640 hasAuthorship W2137231640A5014022727 @default.
- W2137231640 hasAuthorship W2137231640A5014441247 @default.
- W2137231640 hasAuthorship W2137231640A5038256279 @default.
- W2137231640 hasAuthorship W2137231640A5060451599 @default.
- W2137231640 hasBestOaLocation W21372316401 @default.
- W2137231640 hasConcept C104317684 @default.
- W2137231640 hasConcept C112190416 @default.
- W2137231640 hasConcept C153911025 @default.
- W2137231640 hasConcept C17757408 @default.
- W2137231640 hasConcept C24107716 @default.
- W2137231640 hasConcept C552990157 @default.
- W2137231640 hasConcept C55493867 @default.
- W2137231640 hasConcept C67705224 @default.
- W2137231640 hasConcept C70721500 @default.
- W2137231640 hasConcept C86803240 @default.
- W2137231640 hasConceptScore W2137231640C104317684 @default.
- W2137231640 hasConceptScore W2137231640C112190416 @default.
- W2137231640 hasConceptScore W2137231640C153911025 @default.
- W2137231640 hasConceptScore W2137231640C17757408 @default.
- W2137231640 hasConceptScore W2137231640C24107716 @default.
- W2137231640 hasConceptScore W2137231640C552990157 @default.
- W2137231640 hasConceptScore W2137231640C55493867 @default.
- W2137231640 hasConceptScore W2137231640C67705224 @default.
- W2137231640 hasConceptScore W2137231640C70721500 @default.
- W2137231640 hasConceptScore W2137231640C86803240 @default.
- W2137231640 hasIssue "1" @default.
- W2137231640 hasLocation W21372316401 @default.
- W2137231640 hasLocation W21372316402 @default.
- W2137231640 hasLocation W21372316403 @default.
- W2137231640 hasLocation W21372316404 @default.
- W2137231640 hasOpenAccess W2137231640 @default.
- W2137231640 hasPrimaryLocation W21372316401 @default.
- W2137231640 hasRelatedWork W1481221485 @default.
- W2137231640 hasRelatedWork W2044655846 @default.
- W2137231640 hasRelatedWork W2065704027 @default.
- W2137231640 hasRelatedWork W2080216367 @default.
- W2137231640 hasRelatedWork W2088545396 @default.
- W2137231640 hasRelatedWork W2171654274 @default.
- W2137231640 hasRelatedWork W2361846046 @default.
- W2137231640 hasRelatedWork W2803339298 @default.
- W2137231640 hasRelatedWork W2954644365 @default.
- W2137231640 hasRelatedWork W3036199006 @default.
- W2137231640 hasVolume "4" @default.
- W2137231640 isParatext "false" @default.
- W2137231640 isRetracted "false" @default.
- W2137231640 magId "2137231640" @default.
- W2137231640 workType "article" @default.