Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137289976> ?p ?o ?g. }
- W2137289976 endingPage "28763" @default.
- W2137289976 startingPage "28749" @default.
- W2137289976 abstract "The interaction of Bcl-2 family proteins at the mitochondrial outer membrane controls membrane permeability and thereby the apoptotic program. The anti-apoptotic protein Bcl-2 binds to the pro-apoptotic protein Bax to prevent Bax homo-oligomerization required for membrane permeabilization. Here, we used site-specific photocross-linking to map the surfaces of Bax and Bcl-2 that interact in the hetero-complex formed in a Triton X-100 micelle as a membrane surrogate. Heterodimer-specific photoadducts were detected from multiple sites in Bax and Bcl-2. Many of the interaction sites are located in the Bcl-2 homology 3 (BH3) region of Bax and the BH1-3 groove of Bcl-2 that likely form the BH3-BH1-3 groove interface. However, other interaction sites form a second interface that includes helix 6 of Bax and the BH4 region of Bcl-2. Loss-of-function mutations in the BH3 region of Bax and the BH1 region of Bcl-2 disrupted the BH3-BH1-3 interface, as expected. Surprisingly the second interface was also disrupted by these mutations. Similarly, a loss-of-function mutation in the BH4 region of Bcl-2 that forms part of the second interface also disrupted both interfaces. As expected, both kinds of mutation abolished Bcl-2-mediated inhibition of Bax oligomerization in detergent micelles. Therefore, Bcl-2 binds Bax through two interdependent interfaces to inhibit the pro-apoptotic oligomerization of Bax." @default.
- W2137289976 created "2016-06-24" @default.
- W2137289976 creator A5011762197 @default.
- W2137289976 creator A5017366826 @default.
- W2137289976 creator A5039313056 @default.
- W2137289976 creator A5047757182 @default.
- W2137289976 creator A5054898210 @default.
- W2137289976 creator A5066158171 @default.
- W2137289976 creator A5066927062 @default.
- W2137289976 creator A5078080058 @default.
- W2137289976 creator A5079285550 @default.
- W2137289976 date "2010-09-01" @default.
- W2137289976 modified "2023-10-16" @default.
- W2137289976 title "Bcl-2 and Bax Interact via the BH1–3 Groove-BH3 Motif Interface and a Novel Interface Involving the BH4 Motif" @default.
- W2137289976 cites W1530373462 @default.
- W2137289976 cites W1628370182 @default.
- W2137289976 cites W1791183093 @default.
- W2137289976 cites W1825915710 @default.
- W2137289976 cites W1835922068 @default.
- W2137289976 cites W1965071591 @default.
- W2137289976 cites W1965410265 @default.
- W2137289976 cites W1966007015 @default.
- W2137289976 cites W1972835366 @default.
- W2137289976 cites W1976720365 @default.
- W2137289976 cites W1977258127 @default.
- W2137289976 cites W1984636849 @default.
- W2137289976 cites W1987482424 @default.
- W2137289976 cites W1990802782 @default.
- W2137289976 cites W1994611236 @default.
- W2137289976 cites W1995719501 @default.
- W2137289976 cites W1995773170 @default.
- W2137289976 cites W1996271683 @default.
- W2137289976 cites W2000011855 @default.
- W2137289976 cites W2000093730 @default.
- W2137289976 cites W2002919668 @default.
- W2137289976 cites W2007281213 @default.
- W2137289976 cites W2008376982 @default.
- W2137289976 cites W2008841394 @default.
- W2137289976 cites W2012076962 @default.
- W2137289976 cites W2014985938 @default.
- W2137289976 cites W2018972223 @default.
- W2137289976 cites W2019572526 @default.
- W2137289976 cites W2024596511 @default.
- W2137289976 cites W2032049833 @default.
- W2137289976 cites W2033847504 @default.
- W2137289976 cites W2038764386 @default.
- W2137289976 cites W2048372067 @default.
- W2137289976 cites W2058273091 @default.
- W2137289976 cites W2062505601 @default.
- W2137289976 cites W2063362926 @default.
- W2137289976 cites W2065333640 @default.
- W2137289976 cites W2066576270 @default.
- W2137289976 cites W2071917878 @default.
- W2137289976 cites W2072252062 @default.
- W2137289976 cites W2076310395 @default.
- W2137289976 cites W2077319000 @default.
- W2137289976 cites W2085419794 @default.
- W2137289976 cites W2094052642 @default.
- W2137289976 cites W2097007474 @default.
- W2137289976 cites W2099410111 @default.
- W2137289976 cites W2100721448 @default.
- W2137289976 cites W2100762532 @default.
- W2137289976 cites W2106009840 @default.
- W2137289976 cites W2106799894 @default.
- W2137289976 cites W2118691390 @default.
- W2137289976 cites W2134413058 @default.
- W2137289976 cites W2136269288 @default.
- W2137289976 cites W2145955296 @default.
- W2137289976 cites W2147318282 @default.
- W2137289976 cites W2150315067 @default.
- W2137289976 cites W2155235678 @default.
- W2137289976 cites W2156323350 @default.
- W2137289976 cites W80462140 @default.
- W2137289976 doi "https://doi.org/10.1074/jbc.m110.148361" @default.
- W2137289976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2937903" @default.
- W2137289976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20584903" @default.
- W2137289976 hasPublicationYear "2010" @default.
- W2137289976 type Work @default.
- W2137289976 sameAs 2137289976 @default.
- W2137289976 citedByCount "60" @default.
- W2137289976 countsByYear W21372899762012 @default.
- W2137289976 countsByYear W21372899762013 @default.
- W2137289976 countsByYear W21372899762014 @default.
- W2137289976 countsByYear W21372899762015 @default.
- W2137289976 countsByYear W21372899762016 @default.
- W2137289976 countsByYear W21372899762017 @default.
- W2137289976 countsByYear W21372899762018 @default.
- W2137289976 countsByYear W21372899762019 @default.
- W2137289976 countsByYear W21372899762020 @default.
- W2137289976 countsByYear W21372899762021 @default.
- W2137289976 countsByYear W21372899762022 @default.
- W2137289976 countsByYear W21372899762023 @default.
- W2137289976 crossrefType "journal-article" @default.
- W2137289976 hasAuthorship W2137289976A5011762197 @default.
- W2137289976 hasAuthorship W2137289976A5017366826 @default.
- W2137289976 hasAuthorship W2137289976A5039313056 @default.
- W2137289976 hasAuthorship W2137289976A5047757182 @default.
- W2137289976 hasAuthorship W2137289976A5054898210 @default.