FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137290109> ?p ?o ?g. }

• W2137290109 endingPage "1773" @default.
• W2137290109 startingPage "1762" @default.
• W2137290109 abstract "Two enzymes of unknown function from the cog1735 subset of the amidohydrolase superfamily (AHS), LMOf2365_2620 (Lmo2620) from Listeria monocytogenes str. 4b F2365 and Bh0225 from Bacillus halodurans C-125, were cloned, expressed, and purified to homogeneity. The catalytic functions of these two enzymes were interrogated by an integrated strategy encompassing bioinformatics, computational docking to three-dimensional crystal structures, and library screening. The three-dimensional structure of Lmo2620 was determined at a resolution of 1.6 Å with two phosphates and a binuclear zinc center in the active site. The proximal phosphate bridges the binuclear metal center and is 7.1 Å from the distal phosphate. The distal phosphate hydrogen bonds with Lys-242, Lys-244, Arg-275, and Tyr-278. Enzymes within cog1735 of the AHS have previously been shown to catalyze the hydrolysis of substituted lactones. Computational docking of the high-energy intermediate form of the KEGG database to the three-dimensional structure of Lmo2620 highly enriched anionic lactones versus other candidate substrates. The active site structure and the computational docking results suggested that probable substrates would likely include phosphorylated sugar lactones. A small library of diacid sugar lactones and phosphorylated sugar lactones was synthesized and tested for substrate activity with Lmo2620 and Bh0225. Two substrates were identified for these enzymes, d-lyxono-1,4-lactone-5-phosphate and l-ribono-1,4-lactone-5-phosphate. The kcat/Km values for the cobalt-substituted enzymes with these substrates are ∼105 M–1 s–1." @default.
• W2137290109 created "2016-06-24" @default.
• W2137290109 creator A5002277619 @default.
• W2137290109 creator A5006013523 @default.
• W2137290109 creator A5008764291 @default.
• W2137290109 creator A5009419179 @default.
• W2137290109 creator A5014633152 @default.
• W2137290109 creator A5048139641 @default.
• W2137290109 creator A5048470753 @default.
• W2137290109 creator A5061121659 @default.
• W2137290109 creator A5085035303 @default.
• W2137290109 creator A5087297552 @default.
• W2137290109 date "2012-02-15" @default.
• W2137290109 modified "2023-09-26" @default.
• W2137290109 title "Structure-Based Function Discovery of an Enzyme for the Hydrolysis of Phosphorylated Sugar Lactones" @default.
• W2137290109 cites W1479943946 @default.
• W2137290109 cites W1539796472 @default.
• W2137290109 cites W1591005419 @default.
• W2137290109 cites W1964575015 @default.
• W2137290109 cites W1964879478 @default.
• W2137290109 cites W1967496291 @default.
• W2137290109 cites W1972373490 @default.
• W2137290109 cites W1981594977 @default.
• W2137290109 cites W1983960125 @default.
• W2137290109 cites W1984439642 @default.
• W2137290109 cites W1985203726 @default.
• W2137290109 cites W1989773022 @default.
• W2137290109 cites W1996122459 @default.
• W2137290109 cites W2000245381 @default.
• W2137290109 cites W2001733041 @default.
• W2137290109 cites W2001994259 @default.
• W2137290109 cites W2014808512 @default.
• W2137290109 cites W2017391459 @default.
• W2137290109 cites W2018799412 @default.
• W2137290109 cites W2029041735 @default.
• W2137290109 cites W2036660056 @default.
• W2137290109 cites W2041255394 @default.
• W2137290109 cites W2041356453 @default.
• W2137290109 cites W2045245987 @default.
• W2137290109 cites W2054938861 @default.
• W2137290109 cites W2055986456 @default.
• W2137290109 cites W2057003871 @default.
• W2137290109 cites W2068570994 @default.
• W2137290109 cites W2069327965 @default.
• W2137290109 cites W2070333050 @default.
• W2137290109 cites W2074227849 @default.
• W2137290109 cites W2076168781 @default.
• W2137290109 cites W2083676218 @default.
• W2137290109 cites W2084575458 @default.
• W2137290109 cites W2105446670 @default.
• W2137290109 cites W2109817765 @default.
• W2137290109 cites W2113561798 @default.
• W2137290109 cites W2118818078 @default.
• W2137290109 cites W2127924375 @default.
• W2137290109 cites W2128193951 @default.
• W2137290109 cites W2128806579 @default.
• W2137290109 cites W2133790733 @default.
• W2137290109 cites W2142600723 @default.
• W2137290109 cites W2144081223 @default.
• W2137290109 cites W2159482845 @default.
• W2137290109 cites W2160216877 @default.
• W2137290109 cites W2160863902 @default.
• W2137290109 cites W2161732286 @default.
• W2137290109 cites W2162220273 @default.
• W2137290109 cites W2165674132 @default.
• W2137290109 cites W2172281502 @default.
• W2137290109 cites W2180229411 @default.
• W2137290109 cites W2318779613 @default.
• W2137290109 cites W2323630730 @default.
• W2137290109 cites W2323794435 @default.
• W2137290109 cites W4294216483 @default.
• W2137290109 doi "https://doi.org/10.1021/bi201838b" @default.
• W2137290109 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3298459" @default.
• W2137290109 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22313111" @default.
• W2137290109 hasPublicationYear "2012" @default.
• W2137290109 type Work @default.
• W2137290109 sameAs 2137290109 @default.
• W2137290109 citedByCount "15" @default.
• W2137290109 countsByYear W21372901092012 @default.
• W2137290109 countsByYear W21372901092013 @default.
• W2137290109 countsByYear W21372901092014 @default.
• W2137290109 countsByYear W21372901092015 @default.
• W2137290109 countsByYear W21372901092016 @default.
• W2137290109 countsByYear W21372901092017 @default.
• W2137290109 countsByYear W21372901092018 @default.
• W2137290109 crossrefType "journal-article" @default.
• W2137290109 hasAuthorship W2137290109A5002277619 @default.
• W2137290109 hasAuthorship W2137290109A5006013523 @default.
• W2137290109 hasAuthorship W2137290109A5008764291 @default.
• W2137290109 hasAuthorship W2137290109A5009419179 @default.
• W2137290109 hasAuthorship W2137290109A5014633152 @default.
• W2137290109 hasAuthorship W2137290109A5048139641 @default.
• W2137290109 hasAuthorship W2137290109A5048470753 @default.
• W2137290109 hasAuthorship W2137290109A5061121659 @default.
• W2137290109 hasAuthorship W2137290109A5085035303 @default.
• W2137290109 hasAuthorship W2137290109A5087297552 @default.
• W2137290109 hasBestOaLocation W21372901092 @default.
• W2137290109 hasConcept C159110408 @default.

• next