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- W2137291733 abstract "Harmful algal blooms (HABs) occur worldwide in bodies of water that range from ponds to oceans. A few species of algae produce and release lethal toxins that under certain conditions are sufficient to damage human health and marine life and severely impact coastal economies. HABs of Karenia brevis (“red tides”) can develop over hundreds of square miles of open water, such as the Gulf of Mexico, and their control can be challenging. To succeed, control methods for HABs must be based on sound theory, must address the toxic effects of the bloom, and must be applicable on a large scale. This project investigated a chemical approach for mitigating the effects of Florida Harmful Algal Blooms (FHABs) using the amino acid derivative, cysteine methyl ester (CME). Karenia brevis, the alga causing FHABs, releases powerful neurotoxic brevetoxins. CME was evaluated because it can chemically neutralize brevetoxins by molecular binding (Naar, et al, 2007). The specific objective of this research was to determine in vitro the length of time that a single CME treatment could neutralize brevetoxins at the typical Gulf of Mexico summer and winter temperatures of 29°C and 18°C. Sonicated Karenia brevis was combined with artificial seawater, treated with CME, and incubated. The incubated samples were processed using Solid Phase Extraction (SPE) to collect the resulting toxins and derivatives. SPE eluted products were analyzed for brevetoxin content using High Performance Liquid Chromatography–Mass Spectrometry. CME was found to be very effective in neutralizing brevetoxins. A single CME treatment remained 100% effective for 37 days and partially effective for at least an additional seven days. Temperature in the range 18°-29°C did not have a significant influence on how long a treatment was effective (P=0.0978)." @default.
- W2137291733 created "2016-06-24" @default.
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- W2137291733 date "2009-01-01" @default.
- W2137291733 modified "2023-09-27" @default.
- W2137291733 title "Florida Harmful Algal Bloom Mitigation Year Five Neutralizing Brevetoxin Using the Amino Acid Derivative, Cysteine Methyl Ester" @default.
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