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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137381184> ?p ?o ?g. }

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• W2137381184 abstract "In the presence of the anionic surfactant sodium n-dodecyl sulphate (SDS), horseradish peroxidase (HRP) undergoes a deactivation process. Suicide inactivation of horseradish peroxidase by hydrogen peroxide(3 mM) was monitored by the absorbance change in product formation in the catalytic reaction cycle. The progress curve of the catalytic reaction cycle was obtained at 27°C and phosphate buffer 2.5 mM The corresponding kinetic parameters i.e., intact enzyme activity (αi); the apparent rate constant of suicide inactivation by peroxide (ki); and the apparent rate constants of enzyme deactivation by surfactant (kd) were evaluated from the obtained kinetic equations. The experimental data are accounted for by the equations used in this investigation. Addition of SDS to the reaction mixture intensified the inactivation process. The deactivation ability of denaturant could be resolved from the observed inactivation effect of the suicide substrate by applying the proposed model. The results indicate that the deactivation and the inactivation processes are independent of each other." @default.
• W2137381184 created "2016-06-24" @default.
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• W2137381184 date "2005-06-01" @default.
• W2137381184 modified "2023-10-18" @default.
• W2137381184 title "Suicide-peroxide inactivation of horseradish peroxidase in the presence of<i>Sodium n-Dodecyl Sulphate</i>: A study of the enzyme deactivation kinetics" @default.
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• W2137381184 doi "https://doi.org/10.1080/14756360500042846" @default.
• W2137381184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16119200" @default.
• W2137381184 hasPublicationYear "2005" @default.
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