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• W2137432795 abstract "Gregorc A., I. D. Bowen, V. Jenaia, P. Hostnik: Infectious Haematopoietic Necrosis Virus-Induced Cell Death in Fish Cell Culture. Acta Vet. Brno 2005, 74: 279-285. Cell death development was investigated in the epithelioma papulosum cyprini (EPC) cell line after infection with infectious haematopoietic necrosis virus (IHNV). Cytopathic effect (CPE) was evaluated, immunohistochemistry for virus localisation and the development of cell death were studied. Modes of cell death were characterised by staining with TdT-mediated dUTP nick end labelling (TUNEL), and with Annexin V and propidium iodide. CPE was evaluated as 25%, 50%, 75% and 100% at the virus culture infective concentration doses (TCID50/0.1 ml) of 1.25, 6.25, 31.25 and 156.25, respectively, and no CPE was found at the concentration lower than 0.25 TCID50/0.1ml. At the PBS:virus concentration 0.25 TCID50/0.1 ml only a few cells were found anti IHNV antibody positive, and no CPE was observed. In the range of virus concentrations from 6.25 to 156.25 TCID50/0.1 ml all cells were positive. The level of positive cells using the TUNEL technique increased 30% and 55% after the increasing the CPE up to 50% and 75%, respectively. At the higher levels of CPE, all the remaining cells were positive. Infected cells exhibited features of early stage of apoptosis as demonstrated reactivity with annexin V. Apoptotic cells expose external phosphatidylserine residues while preserving membrane integrity. Red fluorescence with propidium iodide is associated with increased levels of necrosis and appeared in cells simultaneously with FITC marker and also alone in vacuolated and enlarged necrotic cells. Apoptosis appears before the pathological changes of necrosis and it is correlated to the spread of infection. The relevance of the current view that IHNV infection includes at least two modes of cell death: apoptosis and necrosis, is discussed." @default.
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• W2137432795 date "2005-01-01" @default.
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• W2137432795 title "Infectious Haematopoietic Necrosis Virus-Induced Cell Death in Fish Cell Culture" @default.
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• W2137432795 doi "https://doi.org/10.2754/avb200574020279" @default.
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