Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137502339> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2137502339 endingPage "1394" @default.
- W2137502339 startingPage "1392" @default.
- W2137502339 abstract "Carousel position-dependent effects on capillary-based LightCycler instruments have been described by Wilhelm et al. (1) and Zuna et al.(2). Both studies found such positional effects to depend on small temperature differences in the thermal chamber of the LightCycler that lead to variable DNA melting efficiencies when amplifying genomic DNA targets with high melting temperatures. These temperature inhomogeneities lead to carousel position-dependent differences in primer binding efficiency and thus to different cycles over threshold (Ct). Both authors postulated that such positional effects would disappear with the instrument’s software version 3.5 that allows constant rotation of the carousel, leveling off possible temperature inhomogeneities in the thermal chamber.However, even when the use of software version 3.5 provided constant carousel rotation, we observed positional effects when amplifying a region of the SNRPN gene in an assay containing 26 reactions (performed on a LightCycler 1.2). Similar effects were observed with the ATP7B gene and the LightCycler Control Kit (Roche), and the effect was even stronger on a LightCycler 1.1 instrument. Position effects were also observed when only 10 reactions were performed. Of special concern is that the …" @default.
- W2137502339 created "2016-06-24" @default.
- W2137502339 creator A5001123080 @default.
- W2137502339 creator A5042381619 @default.
- W2137502339 creator A5045663148 @default.
- W2137502339 creator A5057694645 @default.
- W2137502339 creator A5059520295 @default.
- W2137502339 date "2007-07-01" @default.
- W2137502339 modified "2023-09-26" @default.
- W2137502339 title "Sample Number and Denaturation Time Are Crucial for the Accuracy of Capillary-Based LightCyclers" @default.
- W2137502339 cites W145044592 @default.
- W2137502339 cites W1966108514 @default.
- W2137502339 cites W1994311253 @default.
- W2137502339 cites W2171791043 @default.
- W2137502339 cites W2205582801 @default.
- W2137502339 doi "https://doi.org/10.1373/clinchem.2007.086249" @default.
- W2137502339 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17582157" @default.
- W2137502339 hasPublicationYear "2007" @default.
- W2137502339 type Work @default.
- W2137502339 sameAs 2137502339 @default.
- W2137502339 citedByCount "5" @default.
- W2137502339 countsByYear W21375023392013 @default.
- W2137502339 countsByYear W21375023392015 @default.
- W2137502339 crossrefType "journal-article" @default.
- W2137502339 hasAuthorship W2137502339A5001123080 @default.
- W2137502339 hasAuthorship W2137502339A5042381619 @default.
- W2137502339 hasAuthorship W2137502339A5045663148 @default.
- W2137502339 hasAuthorship W2137502339A5057694645 @default.
- W2137502339 hasAuthorship W2137502339A5059520295 @default.
- W2137502339 hasBestOaLocation W21375023391 @default.
- W2137502339 hasConcept C105795698 @default.
- W2137502339 hasConcept C159985019 @default.
- W2137502339 hasConcept C185592680 @default.
- W2137502339 hasConcept C192562407 @default.
- W2137502339 hasConcept C196806460 @default.
- W2137502339 hasConcept C198531522 @default.
- W2137502339 hasConcept C33923547 @default.
- W2137502339 hasConcept C43617362 @default.
- W2137502339 hasConceptScore W2137502339C105795698 @default.
- W2137502339 hasConceptScore W2137502339C159985019 @default.
- W2137502339 hasConceptScore W2137502339C185592680 @default.
- W2137502339 hasConceptScore W2137502339C192562407 @default.
- W2137502339 hasConceptScore W2137502339C196806460 @default.
- W2137502339 hasConceptScore W2137502339C198531522 @default.
- W2137502339 hasConceptScore W2137502339C33923547 @default.
- W2137502339 hasConceptScore W2137502339C43617362 @default.
- W2137502339 hasIssue "7" @default.
- W2137502339 hasLocation W21375023391 @default.
- W2137502339 hasLocation W21375023392 @default.
- W2137502339 hasOpenAccess W2137502339 @default.
- W2137502339 hasPrimaryLocation W21375023391 @default.
- W2137502339 hasRelatedWork W1526505950 @default.
- W2137502339 hasRelatedWork W197010976 @default.
- W2137502339 hasRelatedWork W1982831602 @default.
- W2137502339 hasRelatedWork W2009467377 @default.
- W2137502339 hasRelatedWork W2046273292 @default.
- W2137502339 hasRelatedWork W2053163850 @default.
- W2137502339 hasRelatedWork W2079554144 @default.
- W2137502339 hasRelatedWork W2080710386 @default.
- W2137502339 hasRelatedWork W2150635844 @default.
- W2137502339 hasRelatedWork W2292754474 @default.
- W2137502339 hasVolume "53" @default.
- W2137502339 isParatext "false" @default.
- W2137502339 isRetracted "false" @default.
- W2137502339 magId "2137502339" @default.
- W2137502339 workType "article" @default.