Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137676886> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2137676886 abstract "Introduction A number of proteins from hyperthermophiles have been produced as the recombinant proteins by introducing cloned genes in Escherichia coli. Some of these recombinant proteins are known to be produced as forms different from those of the natural ones. Recently, we cloned and expressed the gene encoding GDH of the hyperthermophilic archaeon Pyrobaculum islandicum (pis-GDH) in E. coli[1]. The recombinant enzyme was produced as a hexameric form with an extremely low specific activity, and the specific activity of the enzyme purified by a heat treatment was similar to that of the natural one[1]. We have also observed that urea has a significant effect on the proper rearrangement of the enzyme at 37 C. To investigate the structural changes between the low activity form and heat or urea treated form, small-angle X-ray scattering analysis (SAXS) was carried out. As a result, a large difference in the radius and quaternary structure of the molecules was measured by SAXS. Materials and Methods" @default.
- W2137676886 created "2016-06-24" @default.
- W2137676886 creator A5012026574 @default.
- W2137676886 creator A5044308184 @default.
- W2137676886 creator A5070568461 @default.
- W2137676886 creator A5085550157 @default.
- W2137676886 creator A5087777648 @default.
- W2137676886 date "2003-01-01" @default.
- W2137676886 modified "2023-09-25" @default.
- W2137676886 title "Studies on the heat and urea induced rearrangement of subunits measured by small-angle X-ray scattering; the case of hyperthermophilic glutamate dehydrogenase produced in Escherichia coli" @default.
- W2137676886 cites W598956651 @default.
- W2137676886 hasPublicationYear "2003" @default.
- W2137676886 type Work @default.
- W2137676886 sameAs 2137676886 @default.
- W2137676886 citedByCount "0" @default.
- W2137676886 crossrefType "journal-article" @default.
- W2137676886 hasAuthorship W2137676886A5012026574 @default.
- W2137676886 hasAuthorship W2137676886A5044308184 @default.
- W2137676886 hasAuthorship W2137676886A5070568461 @default.
- W2137676886 hasAuthorship W2137676886A5085550157 @default.
- W2137676886 hasAuthorship W2137676886A5087777648 @default.
- W2137676886 hasConcept C104292427 @default.
- W2137676886 hasConcept C104317684 @default.
- W2137676886 hasConcept C120665830 @default.
- W2137676886 hasConcept C121332964 @default.
- W2137676886 hasConcept C131888329 @default.
- W2137676886 hasConcept C170493617 @default.
- W2137676886 hasConcept C181199279 @default.
- W2137676886 hasConcept C185592680 @default.
- W2137676886 hasConcept C191486275 @default.
- W2137676886 hasConcept C2777202666 @default.
- W2137676886 hasConcept C2780365088 @default.
- W2137676886 hasConcept C40767141 @default.
- W2137676886 hasConcept C547475151 @default.
- W2137676886 hasConcept C550995028 @default.
- W2137676886 hasConcept C55428944 @default.
- W2137676886 hasConcept C55493867 @default.
- W2137676886 hasConcept C61174792 @default.
- W2137676886 hasConcept C7927669 @default.
- W2137676886 hasConcept C8010536 @default.
- W2137676886 hasConcept C86803240 @default.
- W2137676886 hasConceptScore W2137676886C104292427 @default.
- W2137676886 hasConceptScore W2137676886C104317684 @default.
- W2137676886 hasConceptScore W2137676886C120665830 @default.
- W2137676886 hasConceptScore W2137676886C121332964 @default.
- W2137676886 hasConceptScore W2137676886C131888329 @default.
- W2137676886 hasConceptScore W2137676886C170493617 @default.
- W2137676886 hasConceptScore W2137676886C181199279 @default.
- W2137676886 hasConceptScore W2137676886C185592680 @default.
- W2137676886 hasConceptScore W2137676886C191486275 @default.
- W2137676886 hasConceptScore W2137676886C2777202666 @default.
- W2137676886 hasConceptScore W2137676886C2780365088 @default.
- W2137676886 hasConceptScore W2137676886C40767141 @default.
- W2137676886 hasConceptScore W2137676886C547475151 @default.
- W2137676886 hasConceptScore W2137676886C550995028 @default.
- W2137676886 hasConceptScore W2137676886C55428944 @default.
- W2137676886 hasConceptScore W2137676886C55493867 @default.
- W2137676886 hasConceptScore W2137676886C61174792 @default.
- W2137676886 hasConceptScore W2137676886C7927669 @default.
- W2137676886 hasConceptScore W2137676886C8010536 @default.
- W2137676886 hasConceptScore W2137676886C86803240 @default.
- W2137676886 hasLocation W21376768861 @default.
- W2137676886 hasOpenAccess W2137676886 @default.
- W2137676886 hasPrimaryLocation W21376768861 @default.
- W2137676886 hasRelatedWork W1489636615 @default.
- W2137676886 hasRelatedWork W1499453995 @default.
- W2137676886 hasRelatedWork W1517713409 @default.
- W2137676886 hasRelatedWork W1975219411 @default.
- W2137676886 hasRelatedWork W1976227410 @default.
- W2137676886 hasRelatedWork W1977157689 @default.
- W2137676886 hasRelatedWork W1994551673 @default.
- W2137676886 hasRelatedWork W2002196487 @default.
- W2137676886 hasRelatedWork W2012725619 @default.
- W2137676886 hasRelatedWork W2020863115 @default.
- W2137676886 hasRelatedWork W2047870553 @default.
- W2137676886 hasRelatedWork W2057893723 @default.
- W2137676886 hasRelatedWork W2073536860 @default.
- W2137676886 hasRelatedWork W2082844098 @default.
- W2137676886 hasRelatedWork W2090695351 @default.
- W2137676886 hasRelatedWork W2105052607 @default.
- W2137676886 hasRelatedWork W2116549131 @default.
- W2137676886 hasRelatedWork W2159056797 @default.
- W2137676886 hasRelatedWork W2166268227 @default.
- W2137676886 hasRelatedWork W2411908615 @default.
- W2137676886 isParatext "false" @default.
- W2137676886 isRetracted "false" @default.
- W2137676886 magId "2137676886" @default.
- W2137676886 workType "article" @default.