Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137792955> ?p ?o ?g. }
- W2137792955 endingPage "650" @default.
- W2137792955 startingPage "642" @default.
- W2137792955 abstract "Asthma exacerbations are caused primarily by viral infections. Antisense and small interfering RNA (siRNA) technologies have gained attention as potential antiasthma and antiviral approaches. In this study we analyzed whether gene silencing of interleukin (IL)-4 expression and respiratory syncytial virus (RSV) replication by RNA interference is able to suppress allergen- and virus-induced responses in a mouse model of virus-induced asthma exacerbation. Knockdown efficacy of IL-4 siRNA molecules was analyzed in the human HEK293T cell line by cotransfection of six different siRNAs with a plasmid carrying mouse IL-4. The most potent siRNA was then used in a mouse model of RSV-induced asthma exacerbation. BALB/c mice were sensitized intraperitoneally with ovalbumin (OVA) and then infected 12 days later intranasally with RSV Long strain (1×106 TCID50/mouse), followed 1 day later by intranasal challenge with OVA for 3 days. Mice were pretreated intranasally three times with either siRNA to IL-4 or GFP control, 2 days before, and on the first two OVA challenge days. siRNAs to RSV or rhinovirus control were inoculated intranasally once, 3 hr before RSV infection. Combined anti-IL-4 and anti-RSV siRNAs were able to significantly reduce total cell counts and eosinophilia in bronchoalveolar lavage fluid, development of airway hyperresponsiveness, and airway inflammation and to downregulate IL-4 mRNA expression and RSV viral RNA, but to upregulate IFN-γ levels in lung tissues. We conclude that anti-helper T cells type 2 and antiviral siRNAs may constitute a new therapeutic approach for treatment of virus induced asthma exacerbations." @default.
- W2137792955 created "2016-06-24" @default.
- W2137792955 creator A5013714559 @default.
- W2137792955 creator A5027331468 @default.
- W2137792955 creator A5031690293 @default.
- W2137792955 creator A5037224969 @default.
- W2137792955 creator A5045371139 @default.
- W2137792955 creator A5048035735 @default.
- W2137792955 creator A5050163445 @default.
- W2137792955 creator A5062099708 @default.
- W2137792955 creator A5064792133 @default.
- W2137792955 date "2014-07-01" @default.
- W2137792955 modified "2023-10-07" @default.
- W2137792955 title "Small Interfering RNAs Targeted to Interleukin-4 and Respiratory Syncytial Virus Reduce Airway Inflammation in a Mouse Model of Virus-Induced Asthma Exacerbation" @default.
- W2137792955 cites W1924334826 @default.
- W2137792955 cites W1930202916 @default.
- W2137792955 cites W1964405262 @default.
- W2137792955 cites W1964779747 @default.
- W2137792955 cites W1980185618 @default.
- W2137792955 cites W1985231378 @default.
- W2137792955 cites W1991730258 @default.
- W2137792955 cites W2003812878 @default.
- W2137792955 cites W2008161734 @default.
- W2137792955 cites W2012678195 @default.
- W2137792955 cites W2014657278 @default.
- W2137792955 cites W2017003565 @default.
- W2137792955 cites W2017014747 @default.
- W2137792955 cites W2023239553 @default.
- W2137792955 cites W2025656480 @default.
- W2137792955 cites W2032904142 @default.
- W2137792955 cites W2046169961 @default.
- W2137792955 cites W2059012483 @default.
- W2137792955 cites W2065176007 @default.
- W2137792955 cites W2067867776 @default.
- W2137792955 cites W2077902297 @default.
- W2137792955 cites W2103090698 @default.
- W2137792955 cites W2103421856 @default.
- W2137792955 cites W2106367530 @default.
- W2137792955 cites W2106867820 @default.
- W2137792955 cites W2114008130 @default.
- W2137792955 cites W2122027002 @default.
- W2137792955 cites W2127818800 @default.
- W2137792955 cites W2128131175 @default.
- W2137792955 cites W2128488922 @default.
- W2137792955 cites W2130145624 @default.
- W2137792955 cites W2132451437 @default.
- W2137792955 cites W2134298234 @default.
- W2137792955 cites W2144836517 @default.
- W2137792955 cites W2148583736 @default.
- W2137792955 cites W2149810044 @default.
- W2137792955 cites W2157566717 @default.
- W2137792955 cites W2164692566 @default.
- W2137792955 cites W2315018741 @default.
- W2137792955 cites W2509274182 @default.
- W2137792955 cites W4251360309 @default.
- W2137792955 cites W4382130656 @default.
- W2137792955 doi "https://doi.org/10.1089/hum.2013.142" @default.
- W2137792955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24655063" @default.
- W2137792955 hasPublicationYear "2014" @default.
- W2137792955 type Work @default.
- W2137792955 sameAs 2137792955 @default.
- W2137792955 citedByCount "32" @default.
- W2137792955 countsByYear W21377929552014 @default.
- W2137792955 countsByYear W21377929552015 @default.
- W2137792955 countsByYear W21377929552016 @default.
- W2137792955 countsByYear W21377929552017 @default.
- W2137792955 countsByYear W21377929552018 @default.
- W2137792955 countsByYear W21377929552019 @default.
- W2137792955 countsByYear W21377929552020 @default.
- W2137792955 countsByYear W21377929552021 @default.
- W2137792955 countsByYear W21377929552022 @default.
- W2137792955 countsByYear W21377929552023 @default.
- W2137792955 crossrefType "journal-article" @default.
- W2137792955 hasAuthorship W2137792955A5013714559 @default.
- W2137792955 hasAuthorship W2137792955A5027331468 @default.
- W2137792955 hasAuthorship W2137792955A5031690293 @default.
- W2137792955 hasAuthorship W2137792955A5037224969 @default.
- W2137792955 hasAuthorship W2137792955A5045371139 @default.
- W2137792955 hasAuthorship W2137792955A5048035735 @default.
- W2137792955 hasAuthorship W2137792955A5050163445 @default.
- W2137792955 hasAuthorship W2137792955A5062099708 @default.
- W2137792955 hasAuthorship W2137792955A5064792133 @default.
- W2137792955 hasConcept C104317684 @default.
- W2137792955 hasConcept C119056186 @default.
- W2137792955 hasConcept C126322002 @default.
- W2137792955 hasConcept C159047783 @default.
- W2137792955 hasConcept C166703698 @default.
- W2137792955 hasConcept C173396325 @default.
- W2137792955 hasConcept C203014093 @default.
- W2137792955 hasConcept C22615655 @default.
- W2137792955 hasConcept C2522874641 @default.
- W2137792955 hasConcept C2777714996 @default.
- W2137792955 hasConcept C2777961210 @default.
- W2137792955 hasConcept C2779278377 @default.
- W2137792955 hasConcept C54009773 @default.
- W2137792955 hasConcept C54355233 @default.
- W2137792955 hasConcept C55493867 @default.
- W2137792955 hasConcept C67705224 @default.