Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137809722> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2137809722 abstract "Studies of regulation of mitochondrial outer membrane selective permeability of cardiac and brain cells in vitro (isolated mitochondria) and in situ (permeabilized cells) condition has revealed remarkable differences between kinetic parameters of respiration activation. This is probably a result of the specific control of voltage dependent anion channel (VDAC) permeability by some cytoskeleton proteins, among them may be some isoforms of tubulin. Our recent results with immunofluorescence and Western blot analysis have shown that possible candidates could be β-tubulin isoforms 2c (IVb) or 4 (IVa). The aim of this study was to construct, express and purify different β-tubulin isoforms and their C-terminal tail truncated recombinants and to investigate their effect on regulation of mitochondrial respiration.Therefore we have designed a GST-tagged β-tubulin isoform constructs to express the recombinant proteins in BL21(DE3)pLys cell line. The proteins were purified in native conditions and utilized to study the kinetics of mitochondrial respiration regulation. The reconstitution experiments with isolated brain mitochondria and GST-TUBB2c or with GST-TUBB4 were carried out. The preliminary results showed that using GST-TUBB2c there were two populations of mitochondria with different properties; the Km value for ADP of the second population of mitochondria was significantly higher, compared to the first population. Therefore, it's possible to conclude that β2c-tubulin is one possible candidate of factor X, which is able to restore the low permeability of VDAC. As the β-tubulin C-terminal tail has been shown to be responsible for selective regulation of VDAC permeability, we have also produced truncated β-tubulin isoforms which lack the C-terminal tail and investigated their effect on mitochondrial respiration. Our results show that β-tubulin 2c isoform is able to restore the diffusion restrictions for ADP which is common for permeabilized cells with oxidative metabolism." @default.
- W2137809722 created "2016-06-24" @default.
- W2137809722 creator A5021091266 @default.
- W2137809722 creator A5036728095 @default.
- W2137809722 creator A5038653004 @default.
- W2137809722 creator A5055777045 @default.
- W2137809722 creator A5072163571 @default.
- W2137809722 creator A5062166971 @default.
- W2137809722 date "2011-02-01" @default.
- W2137809722 modified "2023-09-26" @default.
- W2137809722 title "Regulation of Mitochondrial Respiration by Different Tubulin Isoforms in Vivo" @default.
- W2137809722 doi "https://doi.org/10.1016/j.bpj.2010.12.2702" @default.
- W2137809722 hasPublicationYear "2011" @default.
- W2137809722 type Work @default.
- W2137809722 sameAs 2137809722 @default.
- W2137809722 citedByCount "2" @default.
- W2137809722 countsByYear W21378097222014 @default.
- W2137809722 countsByYear W21378097222020 @default.
- W2137809722 crossrefType "journal-article" @default.
- W2137809722 hasAuthorship W2137809722A5021091266 @default.
- W2137809722 hasAuthorship W2137809722A5036728095 @default.
- W2137809722 hasAuthorship W2137809722A5038653004 @default.
- W2137809722 hasAuthorship W2137809722A5055777045 @default.
- W2137809722 hasAuthorship W2137809722A5062166971 @default.
- W2137809722 hasAuthorship W2137809722A5072163571 @default.
- W2137809722 hasBestOaLocation W21378097221 @default.
- W2137809722 hasConcept C104317684 @default.
- W2137809722 hasConcept C144024400 @default.
- W2137809722 hasConcept C146587185 @default.
- W2137809722 hasConcept C149923435 @default.
- W2137809722 hasConcept C153911025 @default.
- W2137809722 hasConcept C200784783 @default.
- W2137809722 hasConcept C20418707 @default.
- W2137809722 hasConcept C2776415932 @default.
- W2137809722 hasConcept C28859421 @default.
- W2137809722 hasConcept C2908647359 @default.
- W2137809722 hasConcept C53345823 @default.
- W2137809722 hasConcept C547475151 @default.
- W2137809722 hasConcept C55493867 @default.
- W2137809722 hasConcept C84425145 @default.
- W2137809722 hasConcept C86803240 @default.
- W2137809722 hasConcept C95444343 @default.
- W2137809722 hasConceptScore W2137809722C104317684 @default.
- W2137809722 hasConceptScore W2137809722C144024400 @default.
- W2137809722 hasConceptScore W2137809722C146587185 @default.
- W2137809722 hasConceptScore W2137809722C149923435 @default.
- W2137809722 hasConceptScore W2137809722C153911025 @default.
- W2137809722 hasConceptScore W2137809722C200784783 @default.
- W2137809722 hasConceptScore W2137809722C20418707 @default.
- W2137809722 hasConceptScore W2137809722C2776415932 @default.
- W2137809722 hasConceptScore W2137809722C28859421 @default.
- W2137809722 hasConceptScore W2137809722C2908647359 @default.
- W2137809722 hasConceptScore W2137809722C53345823 @default.
- W2137809722 hasConceptScore W2137809722C547475151 @default.
- W2137809722 hasConceptScore W2137809722C55493867 @default.
- W2137809722 hasConceptScore W2137809722C84425145 @default.
- W2137809722 hasConceptScore W2137809722C86803240 @default.
- W2137809722 hasConceptScore W2137809722C95444343 @default.
- W2137809722 hasLocation W21378097221 @default.
- W2137809722 hasOpenAccess W2137809722 @default.
- W2137809722 hasPrimaryLocation W21378097221 @default.
- W2137809722 hasRelatedWork W131363411 @default.
- W2137809722 hasRelatedWork W1520620142 @default.
- W2137809722 hasRelatedWork W1521073795 @default.
- W2137809722 hasRelatedWork W1575666495 @default.
- W2137809722 hasRelatedWork W1973986208 @default.
- W2137809722 hasRelatedWork W1980055846 @default.
- W2137809722 hasRelatedWork W1980448760 @default.
- W2137809722 hasRelatedWork W1986775217 @default.
- W2137809722 hasRelatedWork W1988696131 @default.
- W2137809722 hasRelatedWork W2001495353 @default.
- W2137809722 hasRelatedWork W2046042539 @default.
- W2137809722 hasRelatedWork W2056059867 @default.
- W2137809722 hasRelatedWork W2067939510 @default.
- W2137809722 hasRelatedWork W2069658441 @default.
- W2137809722 hasRelatedWork W2100973201 @default.
- W2137809722 hasRelatedWork W2197230898 @default.
- W2137809722 hasRelatedWork W2272605083 @default.
- W2137809722 hasRelatedWork W2560836156 @default.
- W2137809722 hasRelatedWork W2563354541 @default.
- W2137809722 hasRelatedWork W2774613951 @default.
- W2137809722 isParatext "false" @default.
- W2137809722 isRetracted "false" @default.
- W2137809722 magId "2137809722" @default.
- W2137809722 workType "article" @default.