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• W2137888977 abstract "Stimulation of the homologous recombination DNA-repair pathway via the induction of genomic double-strand breaks (DSBs) by zinc finger nucleases (ZFNs) has been deployed for gene replacement in plant cells. Nonhomologous end joining (NHEJ)-mediated repair of DSBs, on the other hand, has been utilized for the induction of site-specific mutagenesis in plants. Since NHEJ is the dominant DSB repair pathway and can also lead to the capture of foreign DNA molecules, we suggest that it can also be deployed for gene replacement. An acceptor DNA molecule in which a green fluorescent protein (GFP) coding sequence (gfp) was flanked by ZFN recognition sequences was used to produce transgenic target plants. A donor DNA molecule in which a promoterless hygromycin B phosphotransferase-encoding gene (hpt) was flanked by ZFN recognition sequences was constructed. The donor DNA molecule and ZFN expression cassette were delivered into target plants. ZFN-mediated site-specific mutagenesis and complete removal of the GFP coding sequence resulted in the recovery of hygromycin-resistant plants that no longer expressed GFP and in which the hpt gene was unlinked to the acceptor DNA. More importantly, ZFN-mediated digestion of both donor and acceptor DNA molecules resulted in NHEJ-mediated replacement of the gfp with hpt and recovery of hygromycin-resistant plants that no longer expressed GFP and in which the hpt gene was physically linked to the acceptor DNA. Sequence and phenotypical analyses, and transmission of the replacement events to the next generation, confirmed the stability of the NHEJ-induced gene exchange, suggesting its use as a novel method for transgene replacement and gene stacking in plants." @default.
• W2137888977 created "2016-06-24" @default.
• W2137888977 creator A5006209736 @default.
• W2137888977 creator A5064509541 @default.
• W2137888977 creator A5067397681 @default.
• W2137888977 date "2013-03-18" @default.
• W2137888977 modified "2023-10-13" @default.
• W2137888977 title "Nonhomologous End Joining-Mediated Gene Replacement in Plant Cells" @default.
• W2137888977 cites W1511753726 @default.
• W2137888977 cites W1518832976 @default.
• W2137888977 cites W1536706018 @default.
• W2137888977 cites W1538935501 @default.
• W2137888977 cites W163659851 @default.
• W2137888977 cites W1768050488 @default.
• W2137888977 cites W186110447 @default.
• W2137888977 cites W1891040508 @default.
• W2137888977 cites W1967999689 @default.
• W2137888977 cites W1972933969 @default.
• W2137888977 cites W1976019826 @default.
• W2137888977 cites W1977036449 @default.
• W2137888977 cites W1980449150 @default.
• W2137888977 cites W1982969769 @default.
• W2137888977 cites W1986703291 @default.
• W2137888977 cites W1987126503 @default.
• W2137888977 cites W1988813636 @default.
• W2137888977 cites W1990142942 @default.
• W2137888977 cites W1991710875 @default.
• W2137888977 cites W1993673609 @default.
• W2137888977 cites W1995435200 @default.
• W2137888977 cites W1997060995 @default.
• W2137888977 cites W2000685562 @default.
• W2137888977 cites W2000802846 @default.
• W2137888977 cites W2009021661 @default.
• W2137888977 cites W2009043217 @default.
• W2137888977 cites W2009626008 @default.
• W2137888977 cites W2015911939 @default.
• W2137888977 cites W2018438969 @default.
• W2137888977 cites W2021730536 @default.
• W2137888977 cites W2022038512 @default.
• W2137888977 cites W2023003460 @default.
• W2137888977 cites W2024203298 @default.
• W2137888977 cites W2035401705 @default.
• W2137888977 cites W2037473198 @default.
• W2137888977 cites W2037948883 @default.
• W2137888977 cites W2043474702 @default.
• W2137888977 cites W2048277899 @default.
• W2137888977 cites W2063309134 @default.
• W2137888977 cites W2068060682 @default.
• W2137888977 cites W2070730658 @default.
• W2137888977 cites W2080248455 @default.
• W2137888977 cites W2087918598 @default.
• W2137888977 cites W2090614579 @default.
• W2137888977 cites W2096479004 @default.
• W2137888977 cites W2096990623 @default.
• W2137888977 cites W2097685453 @default.
• W2137888977 cites W2100461238 @default.
• W2137888977 cites W2101179888 @default.
• W2137888977 cites W2101655911 @default.
• W2137888977 cites W2104071563 @default.
• W2137888977 cites W2105174770 @default.
• W2137888977 cites W2105216012 @default.
• W2137888977 cites W2105373127 @default.
• W2137888977 cites W2107077624 @default.
• W2137888977 cites W2108733083 @default.
• W2137888977 cites W2109590074 @default.
• W2137888977 cites W2109635885 @default.
• W2137888977 cites W2110260408 @default.
• W2137888977 cites W2111980851 @default.
• W2137888977 cites W2112543742 @default.
• W2137888977 cites W2113568720 @default.
• W2137888977 cites W2116122104 @default.
• W2137888977 cites W2121265940 @default.
• W2137888977 cites W2127120000 @default.
• W2137888977 cites W2132835600 @default.
• W2137888977 cites W2133872029 @default.
• W2137888977 cites W2137082459 @default.
• W2137888977 cites W2137317624 @default.
• W2137888977 cites W2138391420 @default.
• W2137888977 cites W2142150660 @default.
• W2137888977 cites W2146387619 @default.
• W2137888977 cites W2147576780 @default.
• W2137888977 cites W2150798416 @default.
• W2137888977 cites W2150861956 @default.
• W2137888977 cites W2159388002 @default.
• W2137888977 cites W2163987331 @default.
• W2137888977 cites W2165294470 @default.
• W2137888977 cites W2165584051 @default.
• W2137888977 cites W2166850856 @default.
• W2137888977 cites W2168245648 @default.
• W2137888977 cites W2168357216 @default.
• W2137888977 cites W2170674427 @default.
• W2137888977 cites W2400544252 @default.
• W2137888977 cites W2412464107 @default.
• W2137888977 cites W2415765538 @default.
• W2137888977 cites W2444620103 @default.
• W2137888977 doi "https://doi.org/10.1104/pp.112.212910" @default.
• W2137888977 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3641218" @default.
• W2137888977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23509176" @default.

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