Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138008481> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2138008481 endingPage "1479" @default.
- W2138008481 startingPage "1475" @default.
- W2138008481 abstract "The 32P-postlabeling method has found wide application as a sensitive technique for detecting the presence of a broad range of bulky aromatic compounds covalently bound to DNA. In this method, the modified DNA is enzymatically degraded to 3′-mononucleatides and labeled with [32P]-phosphate at the 5′-position using [γ-32P]ATP and T4 polynucleotide kinase. The 32P-labeled DNA digest is then chromatographed in two dimensions on polyethyleneimine-cellulose thin-layer plates. Screenenhanced autoradiography is used to locate the presence of the radiolabeled adducts on the chromatograrn, and the radioactive areas are generally excised and quantitated by liquid scintillation spectrometry. However, on a chromatogram with multiple adducts, it can be difficult to quantitate partially resolved adducts and evaluate background radioactivity levels. We have evaluated the use of storage phosphor imaging techniques to quantitate and map the radioactivity on chromatograms generated by the 32P-postlabeling method. The results showed that storage phosphor imaging was ˜10 times more sensitive than screenenhanced autoradiography at -80°C for the detection of 32P, exhibits a greater linear range of response, has a resolution that compares favorably to film and has a lower background than does liquid scintillation spectrometry. Further, the generation of a digitized record of the distribution and intensity of radioactivity allows for computer-assisted assessment of adduct profiles and can facilitate quantitation of individual adducts and radioactive zones comprised of multiple overlapping adducts in complex chromatograms. Additionally, the permanent record created by the imaging technology permits facile retrospective analysis of samples, whereas with autoradiography and liquid scintillation spectmmetry reanalysis of a replicate sample is required." @default.
- W2138008481 created "2016-06-24" @default.
- W2138008481 creator A5019443047 @default.
- W2138008481 creator A5037697101 @default.
- W2138008481 creator A5085125872 @default.
- W2138008481 creator A5089439321 @default.
- W2138008481 creator A5091443550 @default.
- W2138008481 date "1992-01-01" @default.
- W2138008481 modified "2023-09-25" @default.
- W2138008481 title "Storage phosphor imaging technique for detection and quantitation of DNA adducts measured by the <sup>32</sup>P-postlabeling assay" @default.
- W2138008481 doi "https://doi.org/10.1093/carcin/13.8.1475" @default.
- W2138008481 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1499099" @default.
- W2138008481 hasPublicationYear "1992" @default.
- W2138008481 type Work @default.
- W2138008481 sameAs 2138008481 @default.
- W2138008481 citedByCount "81" @default.
- W2138008481 countsByYear W21380084812013 @default.
- W2138008481 countsByYear W21380084812015 @default.
- W2138008481 countsByYear W21380084812018 @default.
- W2138008481 countsByYear W21380084812019 @default.
- W2138008481 countsByYear W21380084812021 @default.
- W2138008481 crossrefType "journal-article" @default.
- W2138008481 hasAuthorship W2138008481A5019443047 @default.
- W2138008481 hasAuthorship W2138008481A5037697101 @default.
- W2138008481 hasAuthorship W2138008481A5085125872 @default.
- W2138008481 hasAuthorship W2138008481A5089439321 @default.
- W2138008481 hasAuthorship W2138008481A5091443550 @default.
- W2138008481 hasConcept C108204754 @default.
- W2138008481 hasConcept C124385694 @default.
- W2138008481 hasConcept C138268822 @default.
- W2138008481 hasConcept C154945302 @default.
- W2138008481 hasConcept C162356407 @default.
- W2138008481 hasConcept C177322064 @default.
- W2138008481 hasConcept C178790620 @default.
- W2138008481 hasConcept C185592680 @default.
- W2138008481 hasConcept C192562407 @default.
- W2138008481 hasConcept C194097036 @default.
- W2138008481 hasConcept C41008148 @default.
- W2138008481 hasConcept C43617362 @default.
- W2138008481 hasConcept C49040817 @default.
- W2138008481 hasConcept C552990157 @default.
- W2138008481 hasConcept C55493867 @default.
- W2138008481 hasConceptScore W2138008481C108204754 @default.
- W2138008481 hasConceptScore W2138008481C124385694 @default.
- W2138008481 hasConceptScore W2138008481C138268822 @default.
- W2138008481 hasConceptScore W2138008481C154945302 @default.
- W2138008481 hasConceptScore W2138008481C162356407 @default.
- W2138008481 hasConceptScore W2138008481C177322064 @default.
- W2138008481 hasConceptScore W2138008481C178790620 @default.
- W2138008481 hasConceptScore W2138008481C185592680 @default.
- W2138008481 hasConceptScore W2138008481C192562407 @default.
- W2138008481 hasConceptScore W2138008481C194097036 @default.
- W2138008481 hasConceptScore W2138008481C41008148 @default.
- W2138008481 hasConceptScore W2138008481C43617362 @default.
- W2138008481 hasConceptScore W2138008481C49040817 @default.
- W2138008481 hasConceptScore W2138008481C552990157 @default.
- W2138008481 hasConceptScore W2138008481C55493867 @default.
- W2138008481 hasIssue "8" @default.
- W2138008481 hasLocation W21380084811 @default.
- W2138008481 hasLocation W21380084812 @default.
- W2138008481 hasOpenAccess W2138008481 @default.
- W2138008481 hasPrimaryLocation W21380084811 @default.
- W2138008481 hasRelatedWork W1991616557 @default.
- W2138008481 hasRelatedWork W2016472295 @default.
- W2138008481 hasRelatedWork W2026948689 @default.
- W2138008481 hasRelatedWork W2047991101 @default.
- W2138008481 hasRelatedWork W2334792521 @default.
- W2138008481 hasRelatedWork W2341880960 @default.
- W2138008481 hasRelatedWork W2951300725 @default.
- W2138008481 hasRelatedWork W2951566524 @default.
- W2138008481 hasRelatedWork W2953125259 @default.
- W2138008481 hasRelatedWork W2953291986 @default.
- W2138008481 hasVolume "13" @default.
- W2138008481 isParatext "false" @default.
- W2138008481 isRetracted "false" @default.
- W2138008481 magId "2138008481" @default.
- W2138008481 workType "article" @default.