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- W2138049102 abstract "Using the differential centrifugation technique and sequential chemical extraction method,the subcellular distribution and chemical forms of Cd in Salix matsudana grown in nutrient solution were investigated.The results showed that increased Cd levels in the medium caused a significant increase of Cd concentration in all fractions of leaves and roots,most of the accumulated Cd was enriched in the cell wall(FⅠ) of leaves(65%~69%),high Cd concentration was also found in the crude chloroplastic fraction(FⅡ)(14%~22%),the cytosolic fraction(FⅣ) accumulated 6.8%~7.7% of Cd,the fraction containing the lowest level of Cd was the membrane fraction(FⅢ),Cd content in the fraction of leaves decreased in the order:FⅠFⅡFⅣFⅢ.The largest proportion of Cd was located in the walls of root(59%~66%).About 14%~25% of the total Cd contained in the root cells was accumulated in the soluble fraction,Cd concentration of the fractions of root decreased in the order:FⅠ(cell walls)FⅣ(soluble)FⅡ(trophoplast)FⅢ(membrane fraction).The Cd concentration was bound to the different chemical forms,chemical forms of Cd(30%) were extracted with 0.6 mol·L-1 HCl and 1 mol·L-1 NaCl in leaves and roots,the greatest amount of Cd was found in extraction solution of 0.6 mol·L-1 HCl and 1 mol·L-1 NaCl,followed by 2% HAC,and the lowest in extraction of 80% ethanol or dH2O." @default.
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- W2138049102 date "2009-01-01" @default.
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- W2138049102 title "Subcellular distribution and chemical forms of cadmium in Salix matsudana." @default.
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