FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138091393> ?p ?o ?g. }

• W2138091393 endingPage "266" @default.
• W2138091393 startingPage "249" @default.
• W2138091393 abstract "G protein–coupled receptor (GPCR) cascades rely on membrane protein diffusion for signaling and are generally found in spatially constrained subcellular microcompartments. How the geometry of these microcompartments impacts cascade activities, however, is not understood, primarily because of the inability of current live cell–imaging technologies to resolve these small structures. Here, we examine the dynamics of the GPCR rhodopsin within discrete signaling microcompartments of live photoreceptors using a novel high resolution approach. Rhodopsin fused to green fluorescent protein variants, either enhanced green fluorescent protein (EGFP) or the photoactivatable PAGFP (Rho-E/PAGFP), was expressed transgenically in Xenopus laevis rod photoreceptors, and the geometries of light signaling microcompartments formed by lamellar disc membranes and their incisure clefts were resolved by confocal imaging. Multiphoton fluorescence relaxation after photoconversion experiments were then performed with a Ti–sapphire laser focused to the diffraction limit, which produced small sub–cubic micrometer volumes of photoconverted molecules within the discrete microcompartments. A model of molecular diffusion was developed that allows the geometry of the particular compartment being examined to be specified. This was used to interpret the experimental results. Using this unique approach, we showed that rhodopsin mobility across the disc surface was highly heterogeneous. The overall relaxation of Rho-PAGFP fluorescence photoactivated within a microcompartment was biphasic, with a fast phase lasting several seconds and a slow phase of variable duration that required up to several minutes to reach equilibrium. Local Rho-EGFP diffusion within defined compartments was monotonic, however, with an effective lateral diffusion coefficient Dlat = 0.130 ± 0.012 µm2s−1. Comparison of rhodopsin-PAGFP relaxation time courses with model predictions revealed that microcompartment geometry alone may explain both fast local rhodopsin diffusion and its slow equilibration across the greater disc membrane. Our approach has for the first time allowed direct examination of GPCR dynamics within a live cell signaling microcompartment and a quantitative assessment of the impact of compartment geometry on GPCR activity." @default.
• W2138091393 created "2016-06-24" @default.
• W2138091393 creator A5015319838 @default.
• W2138091393 creator A5016430332 @default.
• W2138091393 creator A5043946436 @default.
• W2138091393 creator A5051329326 @default.
• W2138091393 creator A5065520583 @default.
• W2138091393 date "2012-08-13" @default.
• W2138091393 modified "2023-10-18" @default.
• W2138091393 title "Impact of signaling microcompartment geometry on GPCR dynamics in live retinal photoreceptors" @default.
• W2138091393 cites W1520033246 @default.
• W2138091393 cites W1603859820 @default.
• W2138091393 cites W1708405693 @default.
• W2138091393 cites W1964653442 @default.
• W2138091393 cites W1964699887 @default.
• W2138091393 cites W1970028705 @default.
• W2138091393 cites W1972859350 @default.
• W2138091393 cites W1975247969 @default.
• W2138091393 cites W1976243291 @default.
• W2138091393 cites W1985600301 @default.
• W2138091393 cites W1987183910 @default.
• W2138091393 cites W1988097700 @default.
• W2138091393 cites W1988326309 @default.
• W2138091393 cites W1989737071 @default.
• W2138091393 cites W1992004763 @default.
• W2138091393 cites W1993697025 @default.
• W2138091393 cites W1993957715 @default.
• W2138091393 cites W1995497500 @default.
• W2138091393 cites W2013750814 @default.
• W2138091393 cites W2022817606 @default.
• W2138091393 cites W2029347326 @default.
• W2138091393 cites W2031496296 @default.
• W2138091393 cites W2032300143 @default.
• W2138091393 cites W2037870039 @default.
• W2138091393 cites W2038368945 @default.
• W2138091393 cites W2043947202 @default.
• W2138091393 cites W2047508855 @default.
• W2138091393 cites W2049174113 @default.
• W2138091393 cites W2062067536 @default.
• W2138091393 cites W2065747214 @default.
• W2138091393 cites W2077968676 @default.
• W2138091393 cites W2079569513 @default.
• W2138091393 cites W2080904282 @default.
• W2138091393 cites W2081383973 @default.
• W2138091393 cites W2086754614 @default.
• W2138091393 cites W2088689165 @default.
• W2138091393 cites W2100193401 @default.
• W2138091393 cites W2110829301 @default.
• W2138091393 cites W2116348808 @default.
• W2138091393 cites W2118950099 @default.
• W2138091393 cites W2120504835 @default.
• W2138091393 cites W2124386156 @default.
• W2138091393 cites W2125577771 @default.
• W2138091393 cites W2140086723 @default.
• W2138091393 cites W2142103722 @default.
• W2138091393 cites W2150766477 @default.
• W2138091393 cites W2157308120 @default.
• W2138091393 cites W2157765421 @default.
• W2138091393 cites W2163126094 @default.
• W2138091393 cites W2164565944 @default.
• W2138091393 cites W2167460696 @default.
• W2138091393 cites W2188737084 @default.
• W2138091393 doi "https://doi.org/10.1085/jgp.201210818" @default.
• W2138091393 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3434098" @default.
• W2138091393 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22891277" @default.
• W2138091393 hasPublicationYear "2012" @default.
• W2138091393 type Work @default.
• W2138091393 sameAs 2138091393 @default.
• W2138091393 citedByCount "23" @default.
• W2138091393 countsByYear W21380913932012 @default.
• W2138091393 countsByYear W21380913932013 @default.
• W2138091393 countsByYear W21380913932014 @default.
• W2138091393 countsByYear W21380913932015 @default.
• W2138091393 countsByYear W21380913932016 @default.
• W2138091393 countsByYear W21380913932017 @default.
• W2138091393 countsByYear W21380913932018 @default.
• W2138091393 countsByYear W21380913932019 @default.
• W2138091393 countsByYear W21380913932020 @default.
• W2138091393 countsByYear W21380913932021 @default.
• W2138091393 crossrefType "journal-article" @default.
• W2138091393 hasAuthorship W2138091393A5015319838 @default.
• W2138091393 hasAuthorship W2138091393A5016430332 @default.
• W2138091393 hasAuthorship W2138091393A5043946436 @default.
• W2138091393 hasAuthorship W2138091393A5051329326 @default.
• W2138091393 hasAuthorship W2138091393A5065520583 @default.
• W2138091393 hasBestOaLocation W21380913931 @default.
• W2138091393 hasConcept C104317684 @default.
• W2138091393 hasConcept C120665830 @default.
• W2138091393 hasConcept C121332964 @default.
• W2138091393 hasConcept C12554922 @default.
• W2138091393 hasConcept C135285700 @default.
• W2138091393 hasConcept C136009344 @default.
• W2138091393 hasConcept C142613039 @default.
• W2138091393 hasConcept C170493617 @default.
• W2138091393 hasConcept C185592680 @default.
• W2138091393 hasConcept C202033177 @default.
• W2138091393 hasConcept C2780827179 @default.
• W2138091393 hasConcept C55493867 @default.

• next