Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138127002> ?p ?o ?g. }
- W2138127002 endingPage "1241" @default.
- W2138127002 startingPage "1234" @default.
- W2138127002 abstract "Preribosomal particles need to be exported from the nucleus, but their architecture and composition have been unknown. Cryo-EM analysis of pre-60S particles bound to the nuclear export factor Arx1 provides the first structural glance at the immature 60S particle, and the position of Arx1 near the exit tunnel suggests that it may restrict access of factors active during translation. Preribosomal particles evolve in the nucleus through transient interaction with biogenesis factors before export to the cytoplasm. Here, we report the architecture of the late pre-60S particle, purified from Saccharomyces cerevisiae, through Arx1, a nuclear export factor with structural homology to methionine aminopeptidases, or its binding partner Alb1. Cryo-EM reconstruction of the Arx1 particle at 11.9-Å resolution reveals regions of extra density on the pre-60S particle attributed to associated biogenesis factors, confirming the immature state of the nascent subunit. One of these densities could be unambiguously assigned to Arx1. Immunoelectron microscopy and UV cross-linking localize Arx1 close to the ribosomal exit tunnel, in direct contact with ES27, a highly dynamic eukaryotic rRNA expansion segment. The binding of Arx1 at the exit tunnel may position this export factor to prevent premature recruitment of ribosome-associated factors active during translation." @default.
- W2138127002 created "2016-06-24" @default.
- W2138127002 creator A5005320567 @default.
- W2138127002 creator A5010441616 @default.
- W2138127002 creator A5019312718 @default.
- W2138127002 creator A5057468179 @default.
- W2138127002 creator A5066233889 @default.
- W2138127002 creator A5077613316 @default.
- W2138127002 creator A5077907142 @default.
- W2138127002 creator A5089223993 @default.
- W2138127002 date "2012-11-11" @default.
- W2138127002 modified "2023-09-27" @default.
- W2138127002 title "Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel" @default.
- W2138127002 cites W1961791601 @default.
- W2138127002 cites W1966072209 @default.
- W2138127002 cites W1977540027 @default.
- W2138127002 cites W1977979969 @default.
- W2138127002 cites W1981715749 @default.
- W2138127002 cites W1983751391 @default.
- W2138127002 cites W1988018005 @default.
- W2138127002 cites W2001876820 @default.
- W2138127002 cites W2006158980 @default.
- W2138127002 cites W2013051407 @default.
- W2138127002 cites W2029425828 @default.
- W2138127002 cites W2036764500 @default.
- W2138127002 cites W2047024375 @default.
- W2138127002 cites W2048982317 @default.
- W2138127002 cites W2050656609 @default.
- W2138127002 cites W2054142963 @default.
- W2138127002 cites W2056325595 @default.
- W2138127002 cites W2056713455 @default.
- W2138127002 cites W2058904982 @default.
- W2138127002 cites W2060167034 @default.
- W2138127002 cites W2063908091 @default.
- W2138127002 cites W2072834874 @default.
- W2138127002 cites W2080190754 @default.
- W2138127002 cites W2082617067 @default.
- W2138127002 cites W2084824108 @default.
- W2138127002 cites W2087926131 @default.
- W2138127002 cites W2093705507 @default.
- W2138127002 cites W2097438415 @default.
- W2138127002 cites W2105872071 @default.
- W2138127002 cites W2108551329 @default.
- W2138127002 cites W2109184486 @default.
- W2138127002 cites W2112883453 @default.
- W2138127002 cites W2113942795 @default.
- W2138127002 cites W2116622208 @default.
- W2138127002 cites W2117165846 @default.
- W2138127002 cites W2119952251 @default.
- W2138127002 cites W2123023546 @default.
- W2138127002 cites W2123898323 @default.
- W2138127002 cites W2124693268 @default.
- W2138127002 cites W2126431396 @default.
- W2138127002 cites W2126524332 @default.
- W2138127002 cites W2126974066 @default.
- W2138127002 cites W2132629607 @default.
- W2138127002 cites W2136872289 @default.
- W2138127002 cites W2138532429 @default.
- W2138127002 cites W2139137642 @default.
- W2138127002 cites W2142111672 @default.
- W2138127002 cites W2149676146 @default.
- W2138127002 cites W2152722934 @default.
- W2138127002 cites W2153410912 @default.
- W2138127002 cites W2153739292 @default.
- W2138127002 cites W2154170325 @default.
- W2138127002 cites W2154183572 @default.
- W2138127002 cites W2154628692 @default.
- W2138127002 cites W2156235751 @default.
- W2138127002 cites W2163697207 @default.
- W2138127002 cites W2165306451 @default.
- W2138127002 cites W2170546657 @default.
- W2138127002 cites W2916336743 @default.
- W2138127002 doi "https://doi.org/10.1038/nsmb.2438" @default.
- W2138127002 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3678077" @default.
- W2138127002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23142978" @default.
- W2138127002 hasPublicationYear "2012" @default.
- W2138127002 type Work @default.
- W2138127002 sameAs 2138127002 @default.
- W2138127002 citedByCount "104" @default.
- W2138127002 countsByYear W21381270022013 @default.
- W2138127002 countsByYear W21381270022014 @default.
- W2138127002 countsByYear W21381270022015 @default.
- W2138127002 countsByYear W21381270022016 @default.
- W2138127002 countsByYear W21381270022017 @default.
- W2138127002 countsByYear W21381270022018 @default.
- W2138127002 countsByYear W21381270022019 @default.
- W2138127002 countsByYear W21381270022020 @default.
- W2138127002 countsByYear W21381270022021 @default.
- W2138127002 countsByYear W21381270022022 @default.
- W2138127002 countsByYear W21381270022023 @default.
- W2138127002 crossrefType "journal-article" @default.
- W2138127002 hasAuthorship W2138127002A5005320567 @default.
- W2138127002 hasAuthorship W2138127002A5010441616 @default.
- W2138127002 hasAuthorship W2138127002A5019312718 @default.
- W2138127002 hasAuthorship W2138127002A5057468179 @default.
- W2138127002 hasAuthorship W2138127002A5066233889 @default.
- W2138127002 hasAuthorship W2138127002A5077613316 @default.
- W2138127002 hasAuthorship W2138127002A5077907142 @default.