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- W2138346739 abstract "Alternate metabolic pathways for the formation of malonylCoA in the actinomycete Streptomyces aureofaciens are examined. Comparison of the specific activities of pyruvate kinase, pyruvate dehydrogenase, and phosphoenol-pyruvate carboxylase during cultivation, the degree of incorporation of individual radioactive substrates into the tetracycline molecule, and the high randomization of acetate-[2- 14 C], indicate that the malonylCoA used in tetracycline biosynthesis does not appear solely through the carboxylation of acetylCoA. The role of the phosphoenolpyruvate carboxylase and oxaloacetate dehydrogenase systems in the formation of malonylCoA is established, and using radio-GLC, a cell-free preparation of S. aureofaciens mycelium is shown to form malonate from oxaloacetate. The reaction requires HSCoA and NAD + ." @default.
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- W2138346739 date "1977-01-01" @default.
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- W2138346739 title "Alternate pathways of malonylCoA formation in Streptomyces aureofaciens" @default.
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- W2138346739 doi "https://doi.org/10.1016/0031-9422(77)80062-9" @default.
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