FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138386457> ?p ?o ?g. }

• W2138386457 endingPage "34" @default.
• W2138386457 startingPage "326" @default.
• W2138386457 abstract "The Wilms' tumor gene (WT1) is aberrantly over-expressed in leukemic cells. Therefore, we wanted to study the effect of small interfering (siRNA) targeting WT1 in leukemic cells and normal CD34-positive cells with regard to proliferation, induction of apoptosis, and cell differentiation. Furthermore, we wanted to evaluate whether the additional use of BCR-ABL siRNA could increase the anti-leukemic effects of WT1 siRNA in chronic myeloid leukemia (CML) cells.We measured WT1 expression by reverse transcription polymerase chain reaction (RT-PCR) in various cell lines and in leukemic cells from patients, then transfected the cells with WT1-specific and BCR-ABL-specific siRNA before carrying out microarray analysis. We used the tunnel assay to measure apoptotic cells.We observed a reduction of WT1 gene expression, measured by real-time RT-PCR, in all studied cell lines: K-562, Kasumi-1, MV 4-11 and NB-4, as well as in cells of AML and CML patients. The results also demonstrated that WT1 siRNA significantly induced apoptosis and inhibited proliferation in MV4-11 cells, NB-4 cells, Kasumi-1 cells (p<0.01) and in K-562 cells (p<0.02) versus controls. In normal CD34-positive cells, the proliferation was only slightly inhibited (by about 20%) and no induction of apoptosis was found. Combined transfection with WT1 and BCR-ABL siRNA together in K-562 cells increased the inhibition of the rate of proliferation and the rate of induced apoptosis compared to transfection with BCR-ABL siRNA or WT1 siRNA alone (p<0.01). We found that most genes involved in cell signaling and protein metabolism were regulated by the WT1 gene in K-562 cells in a microarray analysis.In conclusion, WT1 might be a suitable target for new therapeutic strategies using siRNAs in leukemic cells." @default.
• W2138386457 created "2016-06-24" @default.
• W2138386457 creator A5020116063 @default.
• W2138386457 creator A5029499905 @default.
• W2138386457 creator A5041775689 @default.
• W2138386457 creator A5046222300 @default.
• W2138386457 creator A5072437531 @default.
• W2138386457 creator A5077872934 @default.
• W2138386457 creator A5083576598 @default.
• W2138386457 date "2005-03-01" @default.
• W2138386457 modified "2023-10-18" @default.
• W2138386457 title "WT1 and BCR-ABL specific small interfering RNA have additive effects in the induction of apoptosis in leukemic cells." @default.
• W2138386457 cites W1498565802 @default.
• W2138386457 cites W1509056501 @default.
• W2138386457 cites W1570247268 @default.
• W2138386457 cites W1611419331 @default.
• W2138386457 cites W1860749330 @default.
• W2138386457 cites W1987363466 @default.
• W2138386457 cites W1989422861 @default.
• W2138386457 cites W2001778516 @default.
• W2138386457 cites W2007607838 @default.
• W2138386457 cites W2014569682 @default.
• W2138386457 cites W2020111601 @default.
• W2138386457 cites W2025936519 @default.
• W2138386457 cites W2045653120 @default.
• W2138386457 cites W2049459265 @default.
• W2138386457 cites W2071482032 @default.
• W2138386457 cites W2075084712 @default.
• W2138386457 cites W2082895851 @default.
• W2138386457 cites W2156593102 @default.
• W2138386457 cites W2159763992 @default.
• W2138386457 cites W2165430941 @default.
• W2138386457 cites W2249166705 @default.
• W2138386457 cites W2313786846 @default.
• W2138386457 cites W2316823625 @default.
• W2138386457 cites W3399256 @default.
• W2138386457 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15749664" @default.
• W2138386457 hasPublicationYear "2005" @default.
• W2138386457 type Work @default.
• W2138386457 sameAs 2138386457 @default.
• W2138386457 citedByCount "14" @default.
• W2138386457 countsByYear W21383864572013 @default.
• W2138386457 countsByYear W21383864572014 @default.
• W2138386457 countsByYear W21383864572015 @default.
• W2138386457 crossrefType "journal-article" @default.
• W2138386457 hasAuthorship W2138386457A5020116063 @default.
• W2138386457 hasAuthorship W2138386457A5029499905 @default.
• W2138386457 hasAuthorship W2138386457A5041775689 @default.
• W2138386457 hasAuthorship W2138386457A5046222300 @default.
• W2138386457 hasAuthorship W2138386457A5072437531 @default.
• W2138386457 hasAuthorship W2138386457A5077872934 @default.
• W2138386457 hasAuthorship W2138386457A5083576598 @default.
• W2138386457 hasConcept C10205521 @default.
• W2138386457 hasConcept C104317684 @default.
• W2138386457 hasConcept C125418893 @default.
• W2138386457 hasConcept C153911025 @default.
• W2138386457 hasConcept C171122931 @default.
• W2138386457 hasConcept C185592680 @default.
• W2138386457 hasConcept C190283241 @default.
• W2138386457 hasConcept C203014093 @default.
• W2138386457 hasConcept C22615655 @default.
• W2138386457 hasConcept C2778461978 @default.
• W2138386457 hasConcept C2778729363 @default.
• W2138386457 hasConcept C28328180 @default.
• W2138386457 hasConcept C42362537 @default.
• W2138386457 hasConcept C43907098 @default.
• W2138386457 hasConcept C502942594 @default.
• W2138386457 hasConcept C54009773 @default.
• W2138386457 hasConcept C54355233 @default.
• W2138386457 hasConcept C55493867 @default.
• W2138386457 hasConcept C62112901 @default.
• W2138386457 hasConcept C62478195 @default.
• W2138386457 hasConcept C81885089 @default.
• W2138386457 hasConcept C86803240 @default.
• W2138386457 hasConcept C95444343 @default.
• W2138386457 hasConceptScore W2138386457C10205521 @default.
• W2138386457 hasConceptScore W2138386457C104317684 @default.
• W2138386457 hasConceptScore W2138386457C125418893 @default.
• W2138386457 hasConceptScore W2138386457C153911025 @default.
• W2138386457 hasConceptScore W2138386457C171122931 @default.
• W2138386457 hasConceptScore W2138386457C185592680 @default.
• W2138386457 hasConceptScore W2138386457C190283241 @default.
• W2138386457 hasConceptScore W2138386457C203014093 @default.
• W2138386457 hasConceptScore W2138386457C22615655 @default.
• W2138386457 hasConceptScore W2138386457C2778461978 @default.
• W2138386457 hasConceptScore W2138386457C2778729363 @default.
• W2138386457 hasConceptScore W2138386457C28328180 @default.
• W2138386457 hasConceptScore W2138386457C42362537 @default.
• W2138386457 hasConceptScore W2138386457C43907098 @default.
• W2138386457 hasConceptScore W2138386457C502942594 @default.
• W2138386457 hasConceptScore W2138386457C54009773 @default.
• W2138386457 hasConceptScore W2138386457C54355233 @default.
• W2138386457 hasConceptScore W2138386457C55493867 @default.
• W2138386457 hasConceptScore W2138386457C62112901 @default.
• W2138386457 hasConceptScore W2138386457C62478195 @default.
• W2138386457 hasConceptScore W2138386457C81885089 @default.
• W2138386457 hasConceptScore W2138386457C86803240 @default.
• W2138386457 hasConceptScore W2138386457C95444343 @default.

• next