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• W2138531432 endingPage "235" @default.
• W2138531432 startingPage "229" @default.
• W2138531432 abstract "A comprehensive synthetic approach, for the localization of the full profile of the continuous antigenic sites on proteins, previously introduced by this laboratory, was applied here to localize the continuous antigenic sites of ragweed allergen, Ra3. The following 10 consecutive peptides, each comprising 15 residues (except for peptide 91–101) and overlapping each of its neighbors by 5 residues, were synthesized and purified: 1–15, 11–25, 21–35, 31–45, 41–55, 51–65, 61–75, 71–85, 81–95 and 91–101. Quantitative radiometric titrations of protein and peptide adsorbents were performed with 125I-labeled anti-Ra3 IgG antibodies from rabbit, outbred mouse and human antisera. The specificity of antibody binding to peptide adsorbents was confirmed by inhibition experiments. These studies established the full profile of antigenic (IgG-binding) sites of Ra3 and permitted comparison with the allergenic (IgE-binding) sites recently localized. It was found that the recognition by IgG antibodies was independent of the host species in which the antibodies were raised. Furthermore, the regions recognized by human IgE antibodies coincided with those recognized by IgG antibodies in three different species. Thus, Ra3 was found to have 4 continuous antigenic sites which occupy the same locations as the allergenic sites." @default.
• W2138531432 created "2016-06-24" @default.
• W2138531432 creator A5003814090 @default.
• W2138531432 creator A5065020704 @default.
• W2138531432 date "1986-01-01" @default.
• W2138531432 modified "2023-10-16" @default.
• W2138531432 title "Antibody recognition of ragweed allergen Ra3: Localization of the full profile of the continuous antigenic sites by synthetic overlapping peptides representing the entire protein chain" @default.
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• W2138531432 doi "https://doi.org/10.1002/eji.1830160304" @default.
• W2138531432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2420608" @default.
• W2138531432 hasPublicationYear "1986" @default.
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