Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138686369> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2138686369 endingPage "1656" @default.
- W2138686369 startingPage "1653" @default.
- W2138686369 abstract "ABSTRACTCopper-zinc superoxide dismutase (CuZnSOD; E.C:1.15.1.1) catalyzes the dismutation of the superoxide radical to hydrogen peroxide and oxygen. In this study, CuZnSOD was isolated from human erythrocytes using DEAE-cellulose chromatography and copper chelate affinity chromatography. The enzyme was purified 196.3 fold with 33.8% efficiency. The molecular weight of CuZnSOD was determined as 20 kDa by SDS-PAGE.Vmax and Km values were determined as 5000 U/mg protein and 3.10−3 mM Xanthine, respectively. Maximum CuZnSOD activity was observed at 15°C. Activation energy was calculated as 16.856 kj/mol and initiation of denaturation temperature was calculated as 19°C. Turnover number (kcat) and catalytic efficiency (kcat/Km) were found to be 1667 s−1 and 5.6 x105 s−1.mM−1 Xanthine−1 respectively.The enzyme was found to have good storage stability as 93.6% of initial activity after 28 days of storage in 50% glycerol solution at -20°C.Keywords: Superoxide DismutasepurificationImmobilized Metal Affinity ChromatographyerythrocyteIminodiacetic Acid Agarose" @default.
- W2138686369 created "2016-06-24" @default.
- W2138686369 creator A5025839107 @default.
- W2138686369 creator A5057254180 @default.
- W2138686369 date "2010-01-01" @default.
- W2138686369 modified "2023-10-09" @default.
- W2138686369 title "Purification of Copper-Zinc Superoxide Dismutase from Human Erythrocytes and Partial Characterization" @default.
- W2138686369 cites W1697966803 @default.
- W2138686369 cites W1775749144 @default.
- W2138686369 cites W1971846633 @default.
- W2138686369 cites W1993822341 @default.
- W2138686369 cites W2000181378 @default.
- W2138686369 cites W2021840827 @default.
- W2138686369 cites W2041604144 @default.
- W2138686369 cites W2100837269 @default.
- W2138686369 cites W2104072424 @default.
- W2138686369 cites W2114443210 @default.
- W2138686369 cites W2135630282 @default.
- W2138686369 cites W2159305277 @default.
- W2138686369 doi "https://doi.org/10.2478/v10133-010-0021-7" @default.
- W2138686369 hasPublicationYear "2010" @default.
- W2138686369 type Work @default.
- W2138686369 sameAs 2138686369 @default.
- W2138686369 citedByCount "7" @default.
- W2138686369 countsByYear W21386863692012 @default.
- W2138686369 countsByYear W21386863692015 @default.
- W2138686369 countsByYear W21386863692016 @default.
- W2138686369 countsByYear W21386863692018 @default.
- W2138686369 countsByYear W21386863692019 @default.
- W2138686369 crossrefType "journal-article" @default.
- W2138686369 hasAuthorship W2138686369A5025839107 @default.
- W2138686369 hasAuthorship W2138686369A5057254180 @default.
- W2138686369 hasBestOaLocation W21386863691 @default.
- W2138686369 hasConcept C13965031 @default.
- W2138686369 hasConcept C178790620 @default.
- W2138686369 hasConcept C179247698 @default.
- W2138686369 hasConcept C181199279 @default.
- W2138686369 hasConcept C185592680 @default.
- W2138686369 hasConcept C2775838275 @default.
- W2138686369 hasConcept C2778248901 @default.
- W2138686369 hasConcept C2779470414 @default.
- W2138686369 hasConcept C2780795997 @default.
- W2138686369 hasConcept C41183919 @default.
- W2138686369 hasConcept C43617362 @default.
- W2138686369 hasConcept C533411734 @default.
- W2138686369 hasConcept C535196362 @default.
- W2138686369 hasConcept C544778455 @default.
- W2138686369 hasConcept C55493867 @default.
- W2138686369 hasConcept C56856141 @default.
- W2138686369 hasConceptScore W2138686369C13965031 @default.
- W2138686369 hasConceptScore W2138686369C178790620 @default.
- W2138686369 hasConceptScore W2138686369C179247698 @default.
- W2138686369 hasConceptScore W2138686369C181199279 @default.
- W2138686369 hasConceptScore W2138686369C185592680 @default.
- W2138686369 hasConceptScore W2138686369C2775838275 @default.
- W2138686369 hasConceptScore W2138686369C2778248901 @default.
- W2138686369 hasConceptScore W2138686369C2779470414 @default.
- W2138686369 hasConceptScore W2138686369C2780795997 @default.
- W2138686369 hasConceptScore W2138686369C41183919 @default.
- W2138686369 hasConceptScore W2138686369C43617362 @default.
- W2138686369 hasConceptScore W2138686369C533411734 @default.
- W2138686369 hasConceptScore W2138686369C535196362 @default.
- W2138686369 hasConceptScore W2138686369C544778455 @default.
- W2138686369 hasConceptScore W2138686369C55493867 @default.
- W2138686369 hasConceptScore W2138686369C56856141 @default.
- W2138686369 hasIssue "1" @default.
- W2138686369 hasLocation W21386863691 @default.
- W2138686369 hasOpenAccess W2138686369 @default.
- W2138686369 hasPrimaryLocation W21386863691 @default.
- W2138686369 hasRelatedWork W1970319427 @default.
- W2138686369 hasRelatedWork W2025098696 @default.
- W2138686369 hasRelatedWork W2037157726 @default.
- W2138686369 hasRelatedWork W2051208451 @default.
- W2138686369 hasRelatedWork W2056261036 @default.
- W2138686369 hasRelatedWork W2059890146 @default.
- W2138686369 hasRelatedWork W2062709989 @default.
- W2138686369 hasRelatedWork W2147934559 @default.
- W2138686369 hasRelatedWork W2403625851 @default.
- W2138686369 hasRelatedWork W4367019171 @default.
- W2138686369 hasVolume "24" @default.
- W2138686369 isParatext "false" @default.
- W2138686369 isRetracted "false" @default.
- W2138686369 magId "2138686369" @default.
- W2138686369 workType "article" @default.