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- W2138695718 abstract "Different statistical methods have been used to classify a gene as differentially expressed in microarray experiments. They usually require a number of experimental observations to be adequately applied. However, many microarray experiments are constrained to low replication designs for different reasons, from financial restrictions to scarcely available RNA samples. Although performed in a high-throughput framework, there are few experimental replicas for each gene to allow the use of traditional or state-of-art statistical methods. In this work, we present a web-based bioinformatics tool that deals with real-life problems concerning low replication experiments. It uses an empirically derived criterion to classify a gene as differentially expressed by combining two widely accepted ideas in microarray analysis: self-self experiments to derive intensity-dependent cutoffs and non-parametric estimation techniques. To help laboratories without a bioinformatics infrastructure, we implemented the tool in a user-friendly website (http://blasto.iq.usp.br/~rvencio/HTself)." @default.
- W2138695718 created "2016-06-24" @default.
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- W2138695718 date "2005-01-01" @default.
- W2138695718 modified "2023-09-26" @default.
- W2138695718 title "HTself: Self-Self Based Statistical Test for Low Replication Microarray Studies" @default.
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- W2138695718 doi "https://doi.org/10.1093/dnares/dsi007" @default.
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