FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138811906> ?p ?o ?g. }

• W2138811906 endingPage "1438" @default.
• W2138811906 startingPage "1427" @default.
• W2138811906 abstract "Transduction of signals initiating motility by extracellular matrix (ECM) molecules differed depending on the type of matrix molecule and whether the ligand was in solution or bound to a substratum. Laminin, fibronectin, and type IV collagen stimulated both chemotaxis and haptotaxis of the A2058 human melanoma cell line. Peak chemotactic responses were reached at 50-200 nM for laminin, 50-100 nM for fibronectin, and 200-370 nM for type IV collagen. Checkerboard analysis of each attractant in solution demonstrated a predominantly directional (chemotactic) response, with a minor chemokinetic component. The cells also migrated in a concentration-dependent manner to insoluble step gradients of substratum-bound attractant (haptotaxis). The haptotactic responses reached maximal levels at coating concentrations of 20 nM for laminin and type IV collagen, and from 30 to 45 nM for fibronectin. Pretreatment of cells with the protein synthesis inhibitor, cycloheximide (5 micrograms/ml), resulted in a 5-30% inhibition of both chemotactic and haptotactic responses to each matrix protein, indicating that de novo protein synthesis was not required for a significant motility response. Pretreatment of cells with 50-500 micrograms/ml of synthetic peptides containing the fibronectin cell-recognition sequence GRGDS resulted in a concentration-dependent inhibition of fibronectin-mediated chemotaxis and haptotaxis (70-80% inhibition compared to control motility); negative control peptide GRGES had only a minimal effect. Neither GRGDS nor GRGES significantly inhibited motility to laminin or type IV collagen. Therefore, these results support a role for the RGD-directed integrin receptor in both types of motility response to fibronectin. After pretreatment with pertussis toxin (PT), chemotactic responses to laminin, fibronectin, and type IV collagen were distinctly different. Chemotaxis to laminin was intermediate in sensitivity; chemotaxis to fibronectin was completely insensitive; and chemotaxis to type IV collagen was profoundly inhibited by PT. In marked contrast to the inhibition of chemotaxis, the hepatotactic responses to all three ligands were unaffected by any of the tested concentrations of PT. High concentrations of cholera toxin (CT; 10 micrograms/ml) or the cAMP analogue, 8-Br-cAMP (0.5 mM), did not significantly affect chemotactic or haptotactic motility to any of the attractant proteins, ruling out the involvement of cAMP in the biochemical pathway initiating motility in these cells. The sensitivity of chemotaxis induced by laminin and type IV collagen, but not fibronectin, to PT indicates the involvement of a PT-sensitive G protein in transduction of the signals initiating motility to soluble laminin and type IV collagen.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2138811906 created "2016-06-24" @default.
• W2138811906 creator A5038321036 @default.
• W2138811906 creator A5049600016 @default.
• W2138811906 creator A5051433481 @default.
• W2138811906 creator A5084068620 @default.
• W2138811906 creator A5086886026 @default.
• W2138811906 date "1990-04-01" @default.
• W2138811906 modified "2023-10-11" @default.
• W2138811906 title "Signal transduction for chemotaxis and haptotaxis by matrix molecules in tumor cells." @default.
• W2138811906 cites W1498888463 @default.
• W2138811906 cites W1513691409 @default.
• W2138811906 cites W1552954655 @default.
• W2138811906 cites W1581180665 @default.
• W2138811906 cites W1606224998 @default.
• W2138811906 cites W1628719417 @default.
• W2138811906 cites W1660697671 @default.
• W2138811906 cites W1874652601 @default.
• W2138811906 cites W1895123769 @default.
• W2138811906 cites W1911780819 @default.
• W2138811906 cites W1967888499 @default.
• W2138811906 cites W1968325967 @default.
• W2138811906 cites W1973279662 @default.
• W2138811906 cites W1975998142 @default.
• W2138811906 cites W1978274173 @default.
• W2138811906 cites W1978834984 @default.
• W2138811906 cites W1986137046 @default.
• W2138811906 cites W1986486534 @default.
• W2138811906 cites W1987568997 @default.
• W2138811906 cites W1988359235 @default.
• W2138811906 cites W1994277288 @default.
• W2138811906 cites W2002038657 @default.
• W2138811906 cites W2005165360 @default.
• W2138811906 cites W2007425850 @default.
• W2138811906 cites W2017382556 @default.
• W2138811906 cites W2024051243 @default.
• W2138811906 cites W2029591777 @default.
• W2138811906 cites W2042099321 @default.
• W2138811906 cites W2052295727 @default.
• W2138811906 cites W2060677356 @default.
• W2138811906 cites W2061752211 @default.
• W2138811906 cites W2068167772 @default.
• W2138811906 cites W2068922572 @default.
• W2138811906 cites W2069439683 @default.
• W2138811906 cites W2073044089 @default.
• W2138811906 cites W2074430638 @default.
• W2138811906 cites W2074511156 @default.
• W2138811906 cites W2080875376 @default.
• W2138811906 cites W2088546959 @default.
• W2138811906 cites W2095774675 @default.
• W2138811906 cites W2096880046 @default.
• W2138811906 cites W2101318220 @default.
• W2138811906 cites W2104685950 @default.
• W2138811906 cites W2105300288 @default.
• W2138811906 cites W2109358961 @default.
• W2138811906 cites W2130053002 @default.
• W2138811906 cites W2140899167 @default.
• W2138811906 cites W2146033738 @default.
• W2138811906 cites W2153222212 @default.
• W2138811906 cites W2156506925 @default.
• W2138811906 cites W2171921813 @default.
• W2138811906 cites W2173768680 @default.
• W2138811906 cites W2179433498 @default.
• W2138811906 cites W67419533 @default.
• W2138811906 doi "https://doi.org/10.1083/jcb.110.4.1427" @default.
• W2138811906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2116083" @default.
• W2138811906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2324200" @default.
• W2138811906 hasPublicationYear "1990" @default.
• W2138811906 type Work @default.
• W2138811906 sameAs 2138811906 @default.
• W2138811906 citedByCount "304" @default.
• W2138811906 countsByYear W21388119062012 @default.
• W2138811906 countsByYear W21388119062013 @default.
• W2138811906 countsByYear W21388119062014 @default.
• W2138811906 countsByYear W21388119062015 @default.
• W2138811906 countsByYear W21388119062016 @default.
• W2138811906 countsByYear W21388119062017 @default.
• W2138811906 countsByYear W21388119062018 @default.
• W2138811906 countsByYear W21388119062019 @default.
• W2138811906 countsByYear W21388119062020 @default.
• W2138811906 countsByYear W21388119062021 @default.
• W2138811906 countsByYear W21388119062022 @default.
• W2138811906 countsByYear W21388119062023 @default.
• W2138811906 crossrefType "journal-article" @default.
• W2138811906 hasAuthorship W2138811906A5038321036 @default.
• W2138811906 hasAuthorship W2138811906A5049600016 @default.
• W2138811906 hasAuthorship W2138811906A5051433481 @default.
• W2138811906 hasAuthorship W2138811906A5084068620 @default.
• W2138811906 hasAuthorship W2138811906A5086886026 @default.
• W2138811906 hasBestOaLocation W21388119061 @default.
• W2138811906 hasConcept C153911025 @default.
• W2138811906 hasConcept C170493617 @default.
• W2138811906 hasConcept C189165786 @default.
• W2138811906 hasConcept C195687474 @default.
• W2138811906 hasConcept C2778128677 @default.
• W2138811906 hasConcept C2779814568 @default.
• W2138811906 hasConcept C3675279 @default.
• W2138811906 hasConcept C54166955 @default.

• next