Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138819935> ?p ?o ?g. }
- W2138819935 endingPage "1084" @default.
- W2138819935 startingPage "1075" @default.
- W2138819935 abstract "The G protein-coupled receptors LGR7 and LGR8 have recently been identified as the primary receptors for the polypeptide hormone relaxin and relaxin-like factors. RT-PCR confirmed the existence of mRNA for both LGR7 and LRG8 in THP-1 cells. Whole cell treatment of THP-1 cells with relaxin produced a biphasic time course in cAMP accumulation, where the first peak appeared as early as 1–2 min with a second peak at 10–20 min. Selective inhibitors for phosphoinositide 3-kinase (PI3K), such as wortmannin and LY294002, showed a dose-dependent inhibition of relaxin-mediated increases in cAMP, specific for the second peak of the relaxin time course. Adenylyl cyclase activation by relaxin in purified plasma membranes from THP-1 cells was not inhibited by LY294002, consistent with a mechanism involving direct stimulation by a Gαs-coupled relaxin receptor. However, reconstitution of membranes with cytosol from THP-1 cells enhanced adenylyl cyclase activity and restored LY294002 sensitivity. In addition, relaxin increased PI3K activity in THP-1 cells. Neither the effects of relaxin nor the inhibition of relaxin by LY294002 was mediated by the activity of phosphodiesterases. Taken together, we show that PI3K is required for the biphasic stimulation of cAMP by relaxin in THP-1 cells and present a novel signal transduction pathway for the activation of adenylyl cyclase by a G protein-coupled receptor." @default.
- W2138819935 created "2016-06-24" @default.
- W2138819935 creator A5038627142 @default.
- W2138819935 creator A5056390040 @default.
- W2138819935 creator A5071126400 @default.
- W2138819935 creator A5083222383 @default.
- W2138819935 date "2003-06-01" @default.
- W2138819935 modified "2023-10-16" @default.
- W2138819935 title "Phosphoinositide 3-Kinase Activity Is Required for Biphasic Stimulation of Cyclic Adenosine 3′,5′-Monophosphate by Relaxin" @default.
- W2138819935 cites W1529396499 @default.
- W2138819935 cites W1557611662 @default.
- W2138819935 cites W1595969520 @default.
- W2138819935 cites W1969231450 @default.
- W2138819935 cites W1969583788 @default.
- W2138819935 cites W1971542049 @default.
- W2138819935 cites W1974186712 @default.
- W2138819935 cites W1978402783 @default.
- W2138819935 cites W198742481 @default.
- W2138819935 cites W1993178097 @default.
- W2138819935 cites W1997092643 @default.
- W2138819935 cites W1998414644 @default.
- W2138819935 cites W2001830950 @default.
- W2138819935 cites W2010559886 @default.
- W2138819935 cites W2012017582 @default.
- W2138819935 cites W2013293486 @default.
- W2138819935 cites W2014984654 @default.
- W2138819935 cites W2018314711 @default.
- W2138819935 cites W2026038641 @default.
- W2138819935 cites W2026470245 @default.
- W2138819935 cites W2041337741 @default.
- W2138819935 cites W2042709942 @default.
- W2138819935 cites W2047826668 @default.
- W2138819935 cites W2049967922 @default.
- W2138819935 cites W2054102046 @default.
- W2138819935 cites W2054403885 @default.
- W2138819935 cites W2056330257 @default.
- W2138819935 cites W2066753814 @default.
- W2138819935 cites W2070732456 @default.
- W2138819935 cites W2080146855 @default.
- W2138819935 cites W2082869755 @default.
- W2138819935 cites W2083891358 @default.
- W2138819935 cites W2084696027 @default.
- W2138819935 cites W2085668133 @default.
- W2138819935 cites W2091904327 @default.
- W2138819935 cites W2092412065 @default.
- W2138819935 cites W2093273780 @default.
- W2138819935 cites W2093343991 @default.
- W2138819935 cites W2094037709 @default.
- W2138819935 cites W2094659070 @default.
- W2138819935 cites W2094755760 @default.
- W2138819935 cites W2099069267 @default.
- W2138819935 cites W2112100215 @default.
- W2138819935 cites W2112134344 @default.
- W2138819935 cites W2125686850 @default.
- W2138819935 cites W2125873842 @default.
- W2138819935 cites W2130080004 @default.
- W2138819935 cites W2137123380 @default.
- W2138819935 cites W2144157272 @default.
- W2138819935 cites W2145387518 @default.
- W2138819935 cites W2161057995 @default.
- W2138819935 cites W2164348457 @default.
- W2138819935 cites W2169623299 @default.
- W2138819935 cites W2399242866 @default.
- W2138819935 cites W2413018742 @default.
- W2138819935 cites W2503588266 @default.
- W2138819935 cites W3146050994 @default.
- W2138819935 doi "https://doi.org/10.1210/me.2002-0284" @default.
- W2138819935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12595573" @default.
- W2138819935 hasPublicationYear "2003" @default.
- W2138819935 type Work @default.
- W2138819935 sameAs 2138819935 @default.
- W2138819935 citedByCount "87" @default.
- W2138819935 countsByYear W21388199352012 @default.
- W2138819935 countsByYear W21388199352013 @default.
- W2138819935 countsByYear W21388199352015 @default.
- W2138819935 countsByYear W21388199352016 @default.
- W2138819935 countsByYear W21388199352017 @default.
- W2138819935 countsByYear W21388199352018 @default.
- W2138819935 countsByYear W21388199352019 @default.
- W2138819935 countsByYear W21388199352020 @default.
- W2138819935 countsByYear W21388199352021 @default.
- W2138819935 countsByYear W21388199352022 @default.
- W2138819935 countsByYear W21388199352023 @default.
- W2138819935 crossrefType "journal-article" @default.
- W2138819935 hasAuthorship W2138819935A5038627142 @default.
- W2138819935 hasAuthorship W2138819935A5056390040 @default.
- W2138819935 hasAuthorship W2138819935A5071126400 @default.
- W2138819935 hasAuthorship W2138819935A5083222383 @default.
- W2138819935 hasBestOaLocation W21388199351 @default.
- W2138819935 hasConcept C126322002 @default.
- W2138819935 hasConcept C134018914 @default.
- W2138819935 hasConcept C163235415 @default.
- W2138819935 hasConcept C170493617 @default.
- W2138819935 hasConcept C184235292 @default.
- W2138819935 hasConcept C24998067 @default.
- W2138819935 hasConcept C2776668983 @default.
- W2138819935 hasConcept C2776912716 @default.
- W2138819935 hasConcept C2776991684 @default.