Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138845922> ?p ?o ?g. }
- W2138845922 endingPage "3380" @default.
- W2138845922 startingPage "3370" @default.
- W2138845922 abstract "Single-stranded DNA constitutes an important early intermediate for homologous recombination and damage-induced cell cycle checkpoint activation. In Saccharomyces cerevisiae, efficient double-strand break (DSB) end resection requires several enzymes; Mre11/Rad50/Xrs2 (MRX) and Sae2 are implicated in the onset of 5'-strand resection, whereas Sgs1/Top3/Rmi1 with Dna2 and Exo1 are involved in extensive resection. However, the molecular events leading to a switch from the MRX/Sae2-dependent initiation to the Exo1- and Dna2-dependent resection remain unclear. Here, we show that MRX recruits Dna2 nuclease to DSB ends. MRX also stimulates recruitment of Exo1 and antagonizes excess binding of the Ku complex to DSB ends. Using resection assay with purified enzymes in vitro, we found that Ku and MRX regulate the nuclease activity of Exo1 in an opposite way. Efficient loading of Dna2 and Exo1 requires neither Sae2 nor Mre11 nuclease activities. However, Mre11 nuclease activity is essential for resection in the absence of extensive resection enzymes. The results provide new insights into how MRX catalyses end resection and recombination initiation." @default.
- W2138845922 created "2016-06-24" @default.
- W2138845922 creator A5024968631 @default.
- W2138845922 creator A5028559592 @default.
- W2138845922 creator A5030317231 @default.
- W2138845922 creator A5034868438 @default.
- W2138845922 creator A5035133412 @default.
- W2138845922 creator A5056589571 @default.
- W2138845922 creator A5059665056 @default.
- W2138845922 creator A5070096888 @default.
- W2138845922 creator A5078360141 @default.
- W2138845922 date "2010-09-10" @default.
- W2138845922 modified "2023-10-11" @default.
- W2138845922 title "Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks" @default.
- W2138845922 cites W1575498014 @default.
- W2138845922 cites W1966018310 @default.
- W2138845922 cites W1966219094 @default.
- W2138845922 cites W1967779036 @default.
- W2138845922 cites W1977829734 @default.
- W2138845922 cites W1978054036 @default.
- W2138845922 cites W1979385111 @default.
- W2138845922 cites W1981372238 @default.
- W2138845922 cites W1987615467 @default.
- W2138845922 cites W1989136612 @default.
- W2138845922 cites W1993026474 @default.
- W2138845922 cites W1997002747 @default.
- W2138845922 cites W2001680897 @default.
- W2138845922 cites W2010711320 @default.
- W2138845922 cites W2019239618 @default.
- W2138845922 cites W2019394964 @default.
- W2138845922 cites W2021845150 @default.
- W2138845922 cites W2023872945 @default.
- W2138845922 cites W2025888720 @default.
- W2138845922 cites W2031292590 @default.
- W2138845922 cites W2031923605 @default.
- W2138845922 cites W2033270381 @default.
- W2138845922 cites W2040392790 @default.
- W2138845922 cites W2043757833 @default.
- W2138845922 cites W2052547134 @default.
- W2138845922 cites W2055532978 @default.
- W2138845922 cites W2058279229 @default.
- W2138845922 cites W2059168612 @default.
- W2138845922 cites W2062284662 @default.
- W2138845922 cites W2064264662 @default.
- W2138845922 cites W2068288569 @default.
- W2138845922 cites W2082981258 @default.
- W2138845922 cites W2083740738 @default.
- W2138845922 cites W2087788529 @default.
- W2138845922 cites W2094020226 @default.
- W2138845922 cites W2102466315 @default.
- W2138845922 cites W2108880649 @default.
- W2138845922 cites W2110912468 @default.
- W2138845922 cites W2123909581 @default.
- W2138845922 cites W2126004462 @default.
- W2138845922 cites W2126492332 @default.
- W2138845922 cites W2129308592 @default.
- W2138845922 cites W2133554835 @default.
- W2138845922 cites W2133701273 @default.
- W2138845922 cites W2137428107 @default.
- W2138845922 cites W2140168282 @default.
- W2138845922 cites W2151606470 @default.
- W2138845922 cites W2162412306 @default.
- W2138845922 cites W2163787826 @default.
- W2138845922 cites W2168588359 @default.
- W2138845922 cites W2170122994 @default.
- W2138845922 cites W4249544688 @default.
- W2138845922 doi "https://doi.org/10.1038/emboj.2010.219" @default.
- W2138845922 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2957216" @default.
- W2138845922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20834227" @default.
- W2138845922 hasPublicationYear "2010" @default.
- W2138845922 type Work @default.
- W2138845922 sameAs 2138845922 @default.
- W2138845922 citedByCount "213" @default.
- W2138845922 countsByYear W21388459222012 @default.
- W2138845922 countsByYear W21388459222013 @default.
- W2138845922 countsByYear W21388459222014 @default.
- W2138845922 countsByYear W21388459222015 @default.
- W2138845922 countsByYear W21388459222016 @default.
- W2138845922 countsByYear W21388459222017 @default.
- W2138845922 countsByYear W21388459222018 @default.
- W2138845922 countsByYear W21388459222019 @default.
- W2138845922 countsByYear W21388459222020 @default.
- W2138845922 countsByYear W21388459222021 @default.
- W2138845922 countsByYear W21388459222022 @default.
- W2138845922 countsByYear W21388459222023 @default.
- W2138845922 crossrefType "journal-article" @default.
- W2138845922 hasAuthorship W2138845922A5024968631 @default.
- W2138845922 hasAuthorship W2138845922A5028559592 @default.
- W2138845922 hasAuthorship W2138845922A5030317231 @default.
- W2138845922 hasAuthorship W2138845922A5034868438 @default.
- W2138845922 hasAuthorship W2138845922A5035133412 @default.
- W2138845922 hasAuthorship W2138845922A5056589571 @default.
- W2138845922 hasAuthorship W2138845922A5059665056 @default.
- W2138845922 hasAuthorship W2138845922A5070096888 @default.
- W2138845922 hasAuthorship W2138845922A5078360141 @default.
- W2138845922 hasBestOaLocation W21388459222 @default.
- W2138845922 hasConcept C102744134 @default.
- W2138845922 hasConcept C104317684 @default.