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- W2138847638 abstract "A method for the attachment of cryptates containing radioactive metal ions to proteins has been developed. The aminocryptand, 4,7,13,16,21,24-hexaoxa-5,6-(4′-aminobenzo)1,10-diazabicyclo[8.8.8]-hexacosane, was complexed with divalent 133Ba and subsequently converted to the isothiocyanatocryptate. The bioconjugate with immunoglobulin G was formed in better than 40% radiochemical yield in a three step procedure. Alternatively, the radiolabeled bioconjugate could be formed by addition of 133Ba to immunoglobulin G that had been reacted with the isothiocyanate derivative of the cryptand. Stability studies indicated the radiolabeled bioconjugate has a half-life of 6 h and 12 h in phosphate buffers of pH=7.4 and pH=9.2, respectively. Attempts to label the immunoglobulin with the corresponding 142Pr cryptate resulted in considerable radioactive colloid formation with no detectable bioconjugate produced." @default.
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- W2138847638 date "1993-04-01" @default.
- W2138847638 modified "2023-09-25" @default.
- W2138847638 title "133Ba cryptate labeled immunoglobulin G" @default.
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- W2138847638 doi "https://doi.org/10.1002/jlcr.2580330409" @default.
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