Matches in SemOpenAlex for { <https://semopenalex.org/work/W2138866523> ?p ?o ?g. }
- W2138866523 endingPage "3918" @default.
- W2138866523 startingPage "3904" @default.
- W2138866523 abstract "The nucleolus is a ubiquitous, mostly spheroidal nuclear structure of all protein-synthesizing cells, with a well-defined functional compartmentalization. Although a number of nonribosomal proteins involved in ribosome formation have been identified, the elements responsible for the shape and internal architecture of nucleoli are still largely unknown. Here, we report the molecular characterization of a novel protein, NO145, which is a major and specific component of a nucleolar cortical skeleton resistant to high salt buffers. The amino acid sequence of this polypeptide with a SDS-PAGE mobility corresponding to M(r) 145,000 has been deduced from a cDNA clone isolated from a Xenopus laevis ovary expression library and defines a polypeptide of 977 amino acids with a calculated mass of 111 kDa, with partial sequence homology to a synaptonemal complex protein, SCP2. Antibodies specific for this protein have allowed its recognition in immunoblots of karyoskeleton-containing fractions of oocytes from different Xenopus species and have revealed its presence in all stages of oogenesis, followed by a specific and rapid degradation during egg formation. Immunolocalization studies at the light and electron microscopic level have shown that protein NO145 is exclusively located in a cage-like cortical structure around the entire nucleolus, consisting of a meshwork of patches and filaments that dissociates upon reduction of divalent cations. We propose that protein NO145 contributes to the assembly of a karyoskeletal structure specific for the nucleolar cortex of the extrachromosomal nucleoli of Xenopus oocytes, and we discuss the possibility that a similar structure is present in other cells and species." @default.
- W2138866523 created "2016-06-24" @default.
- W2138866523 creator A5012785343 @default.
- W2138866523 creator A5015470200 @default.
- W2138866523 creator A5029255990 @default.
- W2138866523 creator A5032317199 @default.
- W2138866523 creator A5052267229 @default.
- W2138866523 creator A5054291480 @default.
- W2138866523 creator A5064987039 @default.
- W2138866523 creator A5083171620 @default.
- W2138866523 creator A5083392770 @default.
- W2138866523 date "2001-12-01" @default.
- W2138866523 modified "2023-10-03" @default.
- W2138866523 title "A Novel Karyoskeletal Protein: Characterization of Protein NO145, the Major Component of Nucleolar Cortical Skeleton in<i>Xenopus</i>Oocytes" @default.
- W2138866523 cites W1489666399 @default.
- W2138866523 cites W1524797524 @default.
- W2138866523 cites W1528109689 @default.
- W2138866523 cites W1586040131 @default.
- W2138866523 cites W1593415742 @default.
- W2138866523 cites W1608387718 @default.
- W2138866523 cites W1816265349 @default.
- W2138866523 cites W1826524642 @default.
- W2138866523 cites W1830164916 @default.
- W2138866523 cites W1876720422 @default.
- W2138866523 cites W1958039167 @default.
- W2138866523 cites W1966521400 @default.
- W2138866523 cites W1970179052 @default.
- W2138866523 cites W1982445736 @default.
- W2138866523 cites W1984330700 @default.
- W2138866523 cites W1985669469 @default.
- W2138866523 cites W1999286258 @default.
- W2138866523 cites W2000362412 @default.
- W2138866523 cites W2003479919 @default.
- W2138866523 cites W2016152543 @default.
- W2138866523 cites W2017301848 @default.
- W2138866523 cites W2020362930 @default.
- W2138866523 cites W2020520526 @default.
- W2138866523 cites W2020525109 @default.
- W2138866523 cites W2022687073 @default.
- W2138866523 cites W2027197597 @default.
- W2138866523 cites W2028052390 @default.
- W2138866523 cites W2030522563 @default.
- W2138866523 cites W2031227962 @default.
- W2138866523 cites W2038520891 @default.
- W2138866523 cites W2039378730 @default.
- W2138866523 cites W2041108688 @default.
- W2138866523 cites W2048653746 @default.
- W2138866523 cites W2054315581 @default.
- W2138866523 cites W2058795573 @default.
- W2138866523 cites W2063358736 @default.
- W2138866523 cites W2064864270 @default.
- W2138866523 cites W2065634301 @default.
- W2138866523 cites W2069359878 @default.
- W2138866523 cites W2074656438 @default.
- W2138866523 cites W2075568133 @default.
- W2138866523 cites W2084815098 @default.
- W2138866523 cites W2086553933 @default.
- W2138866523 cites W2086725260 @default.
- W2138866523 cites W2092515531 @default.
- W2138866523 cites W2093139273 @default.
- W2138866523 cites W2097325653 @default.
- W2138866523 cites W2100021370 @default.
- W2138866523 cites W2104383733 @default.
- W2138866523 cites W2104404316 @default.
- W2138866523 cites W2108388593 @default.
- W2138866523 cites W2110809853 @default.
- W2138866523 cites W2111204676 @default.
- W2138866523 cites W2113051549 @default.
- W2138866523 cites W2116177712 @default.
- W2138866523 cites W2119980510 @default.
- W2138866523 cites W2120754979 @default.
- W2138866523 cites W2124993714 @default.
- W2138866523 cites W2134812217 @default.
- W2138866523 cites W2137131184 @default.
- W2138866523 cites W2138165292 @default.
- W2138866523 cites W2139031970 @default.
- W2138866523 cites W2141676283 @default.
- W2138866523 cites W2144761938 @default.
- W2138866523 cites W2146837416 @default.
- W2138866523 cites W2152121872 @default.
- W2138866523 cites W2153542488 @default.
- W2138866523 cites W2161497672 @default.
- W2138866523 cites W2163390689 @default.
- W2138866523 cites W2168847939 @default.
- W2138866523 cites W2181682893 @default.
- W2138866523 cites W2283961599 @default.
- W2138866523 cites W2334290187 @default.
- W2138866523 cites W2424089066 @default.
- W2138866523 cites W2428494219 @default.
- W2138866523 cites W2463455680 @default.
- W2138866523 cites W2482729339 @default.
- W2138866523 cites W2986463843 @default.
- W2138866523 cites W311391570 @default.
- W2138866523 cites W4248002052 @default.
- W2138866523 cites W4251695548 @default.
- W2138866523 cites W4255731470 @default.
- W2138866523 cites W4294216491 @default.
- W2138866523 cites W80099928 @default.