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- W2138886199 abstract "We previously reported that the type II secreted phospholipase A2 (sPLA2) promoter from positions (−326 to +20) ([−326;+20] promoter) is negatively regulated by two adjacent regulatory elements, C (−210 to −176) and D (−247 to −210). This study examines in greater detail the way in which this negative regulation operates. Successive 5′ deletions of the [−326;+20] type II sPLA2 promoter indicated that the region upstream of position −195 inhibits the transcription activity sixfold in HepG2 cells but not in HeLa cells. Although the whole [−326;−176] region decreased the activity of a heterologous thymidine kinase promoter, this effect was orientation and position sensitive. C/EBPβ, C/EBPα, and C/EBPδ, which bind to element C, prevented the inhibition of promoter activity. Electrophoretic mobility shift experiments identified the binding of NF1-like proteins to the [−225;−218] site, which overlaps an insulin response-like sequence, 5′-TGTTTTG-3′. This sequence bound a factor which also recognized the promoters of the apolipoproteins C-III and A-II. Substitutions preventing the binding of this factor or the NF1-like proteins did not increase the transcription activity, but substitution in the [−217;−204] sequence blocked the transcription inhibition. This sequence did not bind any double-strand binding factor, but its antisense strand is critical for the binding of single-strand binding proteins to the [−232;−191] region. We therefore suggest that these single-strand binding proteins are involved in the inhibitory mechanism." @default.
- W2138886199 created "2016-06-24" @default.
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- W2138886199 date "1997-08-01" @default.
- W2138886199 modified "2023-09-25" @default.
- W2138886199 title "C/EBP Factor Suppression of Inhibition of Type II Secreted Phospholipase A<sub>2</sub> Promoter in HepG2 Cells: Possible Role of Single-Strand Binding Proteins" @default.
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- W2138886199 doi "https://doi.org/10.1128/mcb.17.8.4238" @default.
- W2138886199 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/232277" @default.
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