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• W2138899029 endingPage "2021" @default.
• W2138899029 startingPage "2009" @default.
• W2138899029 abstract "Protein hydrolysis plays an important role during seed germination and post-germination seedling establishment. In Arabidopsis thaliana, cathepsin B-like proteases are encoded by a gene family of three members, but only the AtCathB3 gene is highly induced upon seed germination and at the early post-germination stage. Seeds of a homozygous T-DNA insertion mutant in the AtCathB3 gene have, besides a reduced cathepsin B activity, a slower germination than the wild type. To explore the transcriptional regulation of this gene, we used a combined phylogenetic shadowing approach together with a yeast one-hybrid screening of an arrayed library of approximately 1200 transcription factor open reading frames from Arabidopsis thaliana. We identified a conserved CathB3-element in the promoters of orthologous CathB3 genes within the Brassicaceae species analysed, and, as its DNA-interacting protein, the G-Box Binding Factor1 (GBF1). Transient overexpression of GBF1 together with a PAtCathB3::uidA (β-glucuronidase) construct in tobacco plants revealed a negative effect of GBF1 on expression driven by the AtCathB3 promoter. In stable P35S::GBF1 lines, not only was the expression of the AtCathB3 gene drastically reduced, but a significant slower germination was also observed. In the homozygous knockout mutant for the GBF1 gene, the opposite effect was found. These data indicate that GBF1 is a transcriptional repressor of the AtCathB3 gene and affects the germination kinetics of Arabidopsis thaliana seeds. As AtCathB3 is also expressed during post-germination in the cotyledons, a role for the AtCathB3-like protease in reserve mobilization is also inferred." @default.
• W2138899029 created "2016-06-24" @default.
• W2138899029 creator A5016327390 @default.
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• W2138899029 creator A5025768431 @default.
• W2138899029 creator A5030770140 @default.
• W2138899029 creator A5055781220 @default.
• W2138899029 date "2014-03-05" @default.
• W2138899029 modified "2023-10-02" @default.
• W2138899029 title "The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1" @default.
• W2138899029 cites W1481537657 @default.
• W2138899029 cites W1515612249 @default.
• W2138899029 cites W1539503448 @default.
• W2138899029 cites W1675422558 @default.
• W2138899029 cites W1754967630 @default.
• W2138899029 cites W1868109098 @default.
• W2138899029 cites W1946160338 @default.
• W2138899029 cites W1960968633 @default.
• W2138899029 cites W1967636126 @default.
• W2138899029 cites W1970016978 @default.
• W2138899029 cites W1975436111 @default.
• W2138899029 cites W1980495712 @default.
• W2138899029 cites W1988452478 @default.
• W2138899029 cites W1990699120 @default.
• W2138899029 cites W1992203670 @default.
• W2138899029 cites W1996920241 @default.
• W2138899029 cites W1997274335 @default.
• W2138899029 cites W2001651090 @default.
• W2138899029 cites W2002455376 @default.
• W2138899029 cites W2002560934 @default.
• W2138899029 cites W2004024897 @default.
• W2138899029 cites W2006113133 @default.
• W2138899029 cites W2009393104 @default.
• W2138899029 cites W2018808488 @default.
• W2138899029 cites W2026408174 @default.
• W2138899029 cites W2027448315 @default.
• W2138899029 cites W2029329199 @default.
• W2138899029 cites W2035908878 @default.
• W2138899029 cites W2037928158 @default.
• W2138899029 cites W2043236266 @default.
• W2138899029 cites W2049680164 @default.
• W2138899029 cites W2051628807 @default.
• W2138899029 cites W2062784691 @default.
• W2138899029 cites W2070504948 @default.
• W2138899029 cites W2083699890 @default.
• W2138899029 cites W2084219391 @default.
• W2138899029 cites W2087892194 @default.
• W2138899029 cites W2091448229 @default.
• W2138899029 cites W2106209421 @default.
• W2138899029 cites W2108244474 @default.
• W2138899029 cites W2108733083 @default.
• W2138899029 cites W2110885000 @default.
• W2138899029 cites W2113160145 @default.
• W2138899029 cites W2113206920 @default.
• W2138899029 cites W2114312655 @default.
• W2138899029 cites W2116122104 @default.
• W2138899029 cites W2116352973 @default.
• W2138899029 cites W2117842765 @default.
• W2138899029 cites W2120631497 @default.
• W2138899029 cites W2122431846 @default.
• W2138899029 cites W2122640833 @default.
• W2138899029 cites W2132926880 @default.
• W2138899029 cites W2133307629 @default.
• W2138899029 cites W2133823890 @default.
• W2138899029 cites W2135310874 @default.
• W2138899029 cites W2135995549 @default.
• W2138899029 cites W2137985960 @default.
• W2138899029 cites W2140117843 @default.
• W2138899029 cites W2141292707 @default.
• W2138899029 cites W2142777242 @default.
• W2138899029 cites W2144362290 @default.
• W2138899029 cites W2150076265 @default.
• W2138899029 cites W2152862254 @default.
• W2138899029 cites W2153665389 @default.
• W2138899029 cites W2157009395 @default.
• W2138899029 cites W2160035767 @default.
• W2138899029 cites W2161539176 @default.
• W2138899029 cites W2163439847 @default.
• W2138899029 cites W2163448850 @default.
• W2138899029 cites W2169818221 @default.
• W2138899029 cites W2170641683 @default.
• W2138899029 cites W2171340504 @default.
• W2138899029 cites W2461082345 @default.
• W2138899029 cites W263827941 @default.
• W2138899029 cites W39835563 @default.
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• W2138899029 doi "https://doi.org/10.1093/jxb/eru055" @default.
• W2138899029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3991739" @default.
• W2138899029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24600022" @default.
• W2138899029 hasPublicationYear "2014" @default.
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