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- W2139071750 abstract "Time-lapse cinematography revealed that activated human immunodeficiency virus (HIV)-infected monocytes crawl along surfaces, putting forward a leading pseudopod. Scanning electron micrographs showed monocyte pseudopods associated with spherical structures the size of HIV virions, and transmission electron micrographs revealed HIV virions budding from pseudopods. Filamentous actin (F-actin) was localized by electron microscopy in the pseudopod by heavy meromyosin decoration. Colocalization of F-actin and p24 viral antigen by light microscopy immunofluorescence indicated that F-actin and virus were present on the same pseudopod. These observations indicate that monocytes produce virus from a leading pseudopod. We suggest that HIV secretion at the leading edges of donor monocytes/macrophages may be an efficient way for HIV to infect target cells." @default.
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- W2139071750 date "1996-09-01" @default.
- W2139071750 modified "2023-10-16" @default.
- W2139071750 title "Directional budding of human immunodeficiency virus from monocytes" @default.
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- W2139071750 doi "https://doi.org/10.1128/jvi.70.9.5916-5921.1996" @default.
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