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• W2139074515 abstract "ABSTRACT Three‐week‐old alfalfa ( Medicago sativa ), barley ( Hordeum vulgare ) and maize ( Zea mays ) were exposed for 7 d to 30 µ m of mercury (HgCl 2 ) to characterize the Hg speciation in root, with no symptoms of being poisoned. The largest pool (99%) was associated with the particulate fraction, whereas the soluble fraction (SF) accounted for a minor proportion (<1%). Liquid chromatography coupled with electro‐spray/time of flight mass spectrometry showed that Hg was bound to an array of phytochelatins (PCs) in root SF, which was particularly varied in alfalfa (eight ligands and five stoichiometries), a species that also accumulated homophytochelatins. Spatial localization of Hg in alfalfa roots by microprobe synchrotron X‐ray fluorescence spectroscopy showed that most of the Hg co‐localized with sulphur in the vascular cylinder. Extended X‐ray Absorption Fine Structure (EXAFS) fingerprint fitting revealed that Hg was bound in vivo to organic‐S compounds, i.e. biomolecules containing cysteine. Albeit a minor proportion of total Hg, Hg–PCs complexes in the SF might be important for tolerance to Hg, as was found with Arabidopsis thaliana mutants cad2‐1 (with low glutathione content) and cad1‐3 (unable to synthesize PCs) in comparison with wild type plants. Interestingly, high‐performance liquid chromatography‐electrospray ionization‐time of flight analysis showed that none of these mutants accumulated Hg–biothiol complexes." @default.
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• W2139074515 date "2011-03-15" @default.
• W2139074515 modified "2023-10-16" @default.
• W2139074515 title "Complexation of Hg with phytochelatins is important for plant Hg tolerance" @default.
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• W2139074515 doi "https://doi.org/10.1111/j.1365-3040.2011.02281.x" @default.
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