Matches in SemOpenAlex for { <https://semopenalex.org/work/W2139303052> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2139303052 endingPage "88" @default.
- W2139303052 startingPage "82" @default.
- W2139303052 abstract "Streptomyces lividans acetylxylan esterase removes the 2- or 3-O-acetyl groups from methyl 2,4-di-O-acetyl- and 3,4-di-O-acetyl beta-D-xylopyranoside. When the free hydroxyl group was replaced with a hydrogen or fluorine, the rate of deacetylation was markedly reduced, but regioselectivity was not affected. The regioselectivity of deacetylation was found to be independent of the prevailing conformation of the substrates in solution as determined by 1H-NMR spectroscopy. These observations confirm the importance of the vicinal hydroxyl group and are consistent with our earlier hypothesis that the deacetylation of positions 2 and 3 may involve a common ortho-ester intermediate. Another possible role of the free vicinal hydroxyl group could be the activation of the acyl leaving group in the deacetylation mechanism. Involvement of the free hydroxyl group in the enzyme-substrate binding is not supported by the results of inhibition experiments in which methyl 2,4-di-O-acetyl beta-D-xylopyranoside was used as substrate and its analogues or methyl beta-D-xylopyranoside as inhibitors. The enzyme requires for its efficient action the trans arrangement of the free and acetylated hydroxyl groups at positions 2 and 3." @default.
- W2139303052 created "2016-06-24" @default.
- W2139303052 creator A5048049917 @default.
- W2139303052 creator A5066183156 @default.
- W2139303052 creator A5069627731 @default.
- W2139303052 creator A5079658403 @default.
- W2139303052 date "2003-07-01" @default.
- W2139303052 modified "2023-10-05" @default.
- W2139303052 title "Mode of action of acetylxylan esterase from Streptomyces lividans: a study with deoxy and deoxy-fluoro analogues of acetylated methyl β-d-xylopyranoside" @default.
- W2139303052 cites W1898932130 @default.
- W2139303052 cites W1967797626 @default.
- W2139303052 cites W1979220190 @default.
- W2139303052 cites W1997058046 @default.
- W2139303052 cites W2013181909 @default.
- W2139303052 cites W2022550644 @default.
- W2139303052 cites W2042347963 @default.
- W2139303052 cites W2043403986 @default.
- W2139303052 cites W2043500726 @default.
- W2139303052 cites W2050443108 @default.
- W2139303052 cites W2053363400 @default.
- W2139303052 cites W2080569295 @default.
- W2139303052 cites W2084131741 @default.
- W2139303052 cites W2128175049 @default.
- W2139303052 cites W2921073505 @default.
- W2139303052 cites W2953040318 @default.
- W2139303052 cites W2953361438 @default.
- W2139303052 cites W4211217678 @default.
- W2139303052 doi "https://doi.org/10.1016/s0304-4165(03)00130-2" @default.
- W2139303052 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12880945" @default.
- W2139303052 hasPublicationYear "2003" @default.
- W2139303052 type Work @default.
- W2139303052 sameAs 2139303052 @default.
- W2139303052 citedByCount "24" @default.
- W2139303052 countsByYear W21393030522012 @default.
- W2139303052 countsByYear W21393030522013 @default.
- W2139303052 countsByYear W21393030522014 @default.
- W2139303052 countsByYear W21393030522017 @default.
- W2139303052 countsByYear W21393030522018 @default.
- W2139303052 countsByYear W21393030522020 @default.
- W2139303052 countsByYear W21393030522022 @default.
- W2139303052 countsByYear W21393030522023 @default.
- W2139303052 crossrefType "journal-article" @default.
- W2139303052 hasAuthorship W2139303052A5048049917 @default.
- W2139303052 hasAuthorship W2139303052A5066183156 @default.
- W2139303052 hasAuthorship W2139303052A5069627731 @default.
- W2139303052 hasAuthorship W2139303052A5079658403 @default.
- W2139303052 hasConcept C104317684 @default.
- W2139303052 hasConcept C119157956 @default.
- W2139303052 hasConcept C121349320 @default.
- W2139303052 hasConcept C161790260 @default.
- W2139303052 hasConcept C178790620 @default.
- W2139303052 hasConcept C181199279 @default.
- W2139303052 hasConcept C185592680 @default.
- W2139303052 hasConcept C18903297 @default.
- W2139303052 hasConcept C2777261171 @default.
- W2139303052 hasConcept C2777289219 @default.
- W2139303052 hasConcept C2778565081 @default.
- W2139303052 hasConcept C2781311116 @default.
- W2139303052 hasConcept C55493867 @default.
- W2139303052 hasConcept C59759590 @default.
- W2139303052 hasConcept C71240020 @default.
- W2139303052 hasConcept C86803240 @default.
- W2139303052 hasConceptScore W2139303052C104317684 @default.
- W2139303052 hasConceptScore W2139303052C119157956 @default.
- W2139303052 hasConceptScore W2139303052C121349320 @default.
- W2139303052 hasConceptScore W2139303052C161790260 @default.
- W2139303052 hasConceptScore W2139303052C178790620 @default.
- W2139303052 hasConceptScore W2139303052C181199279 @default.
- W2139303052 hasConceptScore W2139303052C185592680 @default.
- W2139303052 hasConceptScore W2139303052C18903297 @default.
- W2139303052 hasConceptScore W2139303052C2777261171 @default.
- W2139303052 hasConceptScore W2139303052C2777289219 @default.
- W2139303052 hasConceptScore W2139303052C2778565081 @default.
- W2139303052 hasConceptScore W2139303052C2781311116 @default.
- W2139303052 hasConceptScore W2139303052C55493867 @default.
- W2139303052 hasConceptScore W2139303052C59759590 @default.
- W2139303052 hasConceptScore W2139303052C71240020 @default.
- W2139303052 hasConceptScore W2139303052C86803240 @default.
- W2139303052 hasIssue "2" @default.
- W2139303052 hasLocation W21393030521 @default.
- W2139303052 hasLocation W21393030522 @default.
- W2139303052 hasOpenAccess W2139303052 @default.
- W2139303052 hasPrimaryLocation W21393030521 @default.
- W2139303052 hasRelatedWork W2000222180 @default.
- W2139303052 hasRelatedWork W2020930971 @default.
- W2139303052 hasRelatedWork W2022234926 @default.
- W2139303052 hasRelatedWork W2039492421 @default.
- W2139303052 hasRelatedWork W2067626935 @default.
- W2139303052 hasRelatedWork W2083705533 @default.
- W2139303052 hasRelatedWork W2128175049 @default.
- W2139303052 hasRelatedWork W2139303052 @default.
- W2139303052 hasRelatedWork W2325192010 @default.
- W2139303052 hasRelatedWork W2953206905 @default.
- W2139303052 hasVolume "1622" @default.
- W2139303052 isParatext "false" @default.
- W2139303052 isRetracted "false" @default.
- W2139303052 magId "2139303052" @default.
- W2139303052 workType "article" @default.