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- W2139308033 abstract "ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues and immunocytochemistry analyses with antibodies raised against ZAM Gag and Env proteins have shown that all components necessary to assemble ZAM viral particles, i.e., ZAM full-length RNAs and Gag and Env polypeptides, are coexpressed in a small set of follicle cells surrounding the oocyte. By electron microscopy, we have shown that ZAM viral particles are indeed detected in this somatic lineage of cells, which they leave and enter the closely apposed oocyte. Our data provide evidence that the vesicular traffic and yolk granules in the process of vitellogenesis play an important role in ZAM transfer to the oocyte. Our data support the possibility that vitellogenin transfer to the oocyte may help a retroelement pass to the germ line with no need of its envelope product." @default.
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- W2139308033 date "2000-11-15" @default.
- W2139308033 modified "2023-10-05" @default.
- W2139308033 title "Life Cycle of an Endogenous Retrovirus, <i>ZAM</i> , in <i>Drosophila melanogaster</i>" @default.
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- W2139308033 doi "https://doi.org/10.1128/jvi.74.22.10658-10669.2000" @default.
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