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• W2139845705 abstract "The in vitro interactions of Yersinia enterocolitica, Salmonella typhimurium and Escherichia coli with polarized human colonic carcinoma (Caco-2) cells are described. Invasion of a confluent Caco-2 cell monolayer by Yersinia and Salmonella took place within 4 h after contact, which was in marked contrast to E. coli which did not invade Caco-2 cells. Cytoplasmic extrusions developed on the apical membrane and indicated the site of entrance of bacteria into the Caco-2 cells. Intracellular Yersinia and Salmonella were surrounded by a vacuolar membrane. Single as well as multiple bacteria were enclosed within a single vacuole. At 6 h after contact some of the intracellular yersiniae were found free in the cytoplasm. Furthermore, morphological signs of degeneration of Caco-2 cells such as vacuolization and autophagy were observed. Caco-2 cells infected with Salmonella also showed degenerative changes but the salmonellae resided within membrane-bound vacuoles in contrast to Yersinia. These observations are in contrast to those described for the invasion of other cells lines (not derived from intestinal epithelium) by Yersinia and may reflect more closely the interactions between Yersinia and the intestinal epithelium during gastrointestinal infection." @default.
• W2139845705 created "2016-06-24" @default.
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• W2139845705 date "1995-10-01" @default.
• W2139845705 modified "2023-09-27" @default.
• W2139845705 title "Interactions of Yersinia enterocolitica with polarized human intestinal Caco-2 cells" @default.
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• W2139845705 doi "https://doi.org/10.1007/bf00224348" @default.
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• W2139845705 hasPublicationYear "1995" @default.
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