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- W2139867285 abstract "We have examined the expression of the ovine clusterin gene in the sheep pituitary gland, with the aim of determining its site of synthesis in this tissue. Northern blotting analysis of extracted polyadenylated RNA, using a (32)P-labelled rat clusterin cDNA probe, detected the greatest amounts of clusterin mRNA in the anterior part of dissected pituitary glands. In situ hybridisation studies showed clusterin mRNA in anterior and intermediate pituitary cells, with lower amounts in vascular endothelium and posterior pituicytes. Clusterin protein, detected by immunohistochemistry, was observed in some single secretory cells, within the capillary lumen and in cells around capillaries in the anterior and intermediate lobes, but no immunoreactivity was observed in posterior pituitary tissue. The pattern of clusterin expression in anterior and intermediate pituitary cells suggests possible roles for the protein in secretory cell turnover and/or hormone secretion or lipid uptake. Clusterin does not appear to be involved in ovine posterior pituitary hormone neurosecretion." @default.
- W2139867285 created "2016-06-24" @default.
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- W2139867285 date "1999-10-01" @default.
- W2139867285 modified "2023-10-06" @default.
- W2139867285 title "Clusterin is expressed in the anterior and intermediate lobes, but not in the posterior pituitary of sheep" @default.
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- W2139867285 doi "https://doi.org/10.1677/jme.0.0230199" @default.
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