FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2139878468> ?p ?o ?g. }

• W2139878468 endingPage "720" @default.
• W2139878468 startingPage "713" @default.
• W2139878468 abstract "The functions of RNA molecules are intimately linked to their ability to fold into complex secondary and tertiary structures. Thus, understanding how these molecules fold is essential to determining how they function. Current methods for investigating RNA structure often use small molecules, enzymes, or ions that cleave or modify the RNA in a solvent-accessible manner. While these methods have been invaluable to understanding RNA structure, they can be fairly labor intensive and often focus on short regions of single RNAs. Here we present a new method (Mod-seq) and data analysis pipeline (Mod-seeker) for assaying the structure of RNAs by high-throughput sequencing. This technique can be utilized both in vivo and in vitro, with any small molecule that modifies RNA and consequently impedes reverse transcriptase. As proof-of-principle, we used dimethyl sulfate (DMS) to probe the in vivo structure of total cellular RNAs in Saccharomyces cerevisiae. Mod-seq analysis simultaneously revealed secondary structural information for all four ribosomal RNAs and 32 additional noncoding RNAs. We further show that Mod-seq can be used to detect structural changes in 5.8S and 25S rRNAs in the absence of ribosomal protein L26, correctly identifying its binding site on the ribosome. While this method is applicable to RNAs of any length, its high-throughput nature makes Mod-seq ideal for studying long RNAs and complex RNA mixtures." @default.
• W2139878468 created "2016-06-24" @default.
• W2139878468 creator A5049964773 @default.
• W2139878468 creator A5058623063 @default.
• W2139878468 creator A5088486292 @default.
• W2139878468 creator A5088627617 @default.
• W2139878468 creator A5088881927 @default.
• W2139878468 date "2014-03-24" @default.
• W2139878468 modified "2023-10-16" @default.
• W2139878468 title "Mod-seq: high-throughput sequencing for chemical probing of RNA structure" @default.
• W2139878468 cites W1259363667 @default.
• W2139878468 cites W1486369891 @default.
• W2139878468 cites W1492281244 @default.
• W2139878468 cites W1966547604 @default.
• W2139878468 cites W2003993220 @default.
• W2139878468 cites W2006486495 @default.
• W2139878468 cites W2007205568 @default.
• W2139878468 cites W2018015544 @default.
• W2139878468 cites W2026181790 @default.
• W2139878468 cites W2026358434 @default.
• W2139878468 cites W2027139827 @default.
• W2139878468 cites W2032136448 @default.
• W2139878468 cites W2038598202 @default.
• W2139878468 cites W2046335009 @default.
• W2139878468 cites W2060167034 @default.
• W2139878468 cites W2064575247 @default.
• W2139878468 cites W2065841577 @default.
• W2139878468 cites W2069779808 @default.
• W2139878468 cites W2071849521 @default.
• W2139878468 cites W2071883352 @default.
• W2139878468 cites W2076091642 @default.
• W2139878468 cites W2086157203 @default.
• W2139878468 cites W2112749414 @default.
• W2139878468 cites W2120974004 @default.
• W2139878468 cites W2122695112 @default.
• W2139878468 cites W2124985265 @default.
• W2139878468 cites W2142056356 @default.
• W2139878468 cites W2161885693 @default.
• W2139878468 cites W2167557255 @default.
• W2139878468 cites W52783179 @default.
• W2139878468 doi "https://doi.org/10.1261/rna.042218.113" @default.
• W2139878468 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3988572" @default.
• W2139878468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24664469" @default.
• W2139878468 hasPublicationYear "2014" @default.
• W2139878468 type Work @default.
• W2139878468 sameAs 2139878468 @default.
• W2139878468 citedByCount "162" @default.
• W2139878468 countsByYear W21398784682014 @default.
• W2139878468 countsByYear W21398784682015 @default.
• W2139878468 countsByYear W21398784682016 @default.
• W2139878468 countsByYear W21398784682017 @default.
• W2139878468 countsByYear W21398784682018 @default.
• W2139878468 countsByYear W21398784682019 @default.
• W2139878468 countsByYear W21398784682020 @default.
• W2139878468 countsByYear W21398784682021 @default.
• W2139878468 countsByYear W21398784682022 @default.
• W2139878468 countsByYear W21398784682023 @default.
• W2139878468 crossrefType "journal-article" @default.
• W2139878468 hasAuthorship W2139878468A5049964773 @default.
• W2139878468 hasAuthorship W2139878468A5058623063 @default.
• W2139878468 hasAuthorship W2139878468A5088486292 @default.
• W2139878468 hasAuthorship W2139878468A5088627617 @default.
• W2139878468 hasAuthorship W2139878468A5088881927 @default.
• W2139878468 hasBestOaLocation W21398784681 @default.
• W2139878468 hasConcept C104317684 @default.
• W2139878468 hasConcept C105130999 @default.
• W2139878468 hasConcept C126142528 @default.
• W2139878468 hasConcept C153957851 @default.
• W2139878468 hasConcept C179255354 @default.
• W2139878468 hasConcept C194993378 @default.
• W2139878468 hasConcept C54355233 @default.
• W2139878468 hasConcept C67705224 @default.
• W2139878468 hasConcept C67905577 @default.
• W2139878468 hasConcept C70721500 @default.
• W2139878468 hasConcept C86803240 @default.
• W2139878468 hasConcept C88478588 @default.
• W2139878468 hasConceptScore W2139878468C104317684 @default.
• W2139878468 hasConceptScore W2139878468C105130999 @default.
• W2139878468 hasConceptScore W2139878468C126142528 @default.
• W2139878468 hasConceptScore W2139878468C153957851 @default.
• W2139878468 hasConceptScore W2139878468C179255354 @default.
• W2139878468 hasConceptScore W2139878468C194993378 @default.
• W2139878468 hasConceptScore W2139878468C54355233 @default.
• W2139878468 hasConceptScore W2139878468C67705224 @default.
• W2139878468 hasConceptScore W2139878468C67905577 @default.
• W2139878468 hasConceptScore W2139878468C70721500 @default.
• W2139878468 hasConceptScore W2139878468C86803240 @default.
• W2139878468 hasConceptScore W2139878468C88478588 @default.
• W2139878468 hasIssue "5" @default.
• W2139878468 hasLocation W21398784681 @default.
• W2139878468 hasLocation W21398784682 @default.
• W2139878468 hasLocation W21398784683 @default.
• W2139878468 hasLocation W21398784684 @default.
• W2139878468 hasOpenAccess W2139878468 @default.
• W2139878468 hasPrimaryLocation W21398784681 @default.
• W2139878468 hasRelatedWork W2011940233 @default.
• W2139878468 hasRelatedWork W2055114431 @default.
• W2139878468 hasRelatedWork W2096327663 @default.

• next