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- W2139953625 abstract "Loop-mediated isothermal amplification (LAMP) is an isothermal amplification technique that can be undertaken with minimal equipment to obtain amplification of target DNA within 30 minutes. A range of assays for specific 16Sr phytoplasma groups, which when combined with rapid DNA extraction techniques can result in detection of the phytoplasma in plant material within 1 hour of sampling was developed. A range of alternative methods are available for detection of the amplification product including incorporation of hydroxyl napthol blue into the reagent mix, agarose gel electrophoresis and real-time detection systems. The ad- vantage of the real-time method is that tubes do not have to be opened, minimising the risk of contamination of samples." @default.
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- W2139953625 date "2011-01-01" @default.
- W2139953625 modified "2023-09-27" @default.
- W2139953625 title "Development of rapid in-field loop-mediated isothermal amplification (LAMP) assays for phytoplasmas" @default.
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