Matches in SemOpenAlex for { <https://semopenalex.org/work/W2139968664> ?p ?o ?g. }
- W2139968664 endingPage "101" @default.
- W2139968664 startingPage "92" @default.
- W2139968664 abstract "We have successfully established mixed glial cell primary cultures prepared from individual fetal human brains (15–18 weeks' gestation in age). Cultures were maintained for as long as 3 months in either 10% fetal calf serum (FCS) or serum-free chemically defined medium (CDM). By morphological and immunohistochemical criteria, the precursor cell for human oligodendrocytes (O-2A cell) was identified. This cell exhibited the bipolar morphology and A2B5-positive (A2B5+) immunoreactivity typical of the O-2A precursor cell. With time in culture, cells possessing a stellate morphology appeared, some of which stained with the O4 antibody, indicative of cell differentiation in the oligodendroglial lineage. At yet older culture age, arborized cells bearing the O1 (galactocerebroside, GC) immunohistochemical marker and displaying the morphological characteristics typical of more mature oligodendrocytes were found, confirming their oligodendroglial identity. Oligodendroglial differentiation was supported best by CDM rather than FCS. To complement these observations, double immunofluorescent studies were performed on parietal sections from human fetal brains at 20 to 22 weeks of gestation. Bipolar A2B5+, multipolar A2B5+/O4+, and arborized A2B5−/O1+ cells were found, thus confirming the presence of oligodendrocytes in human fetal brain at this stage of prenatal development and consistent with the observations made in cell culture." @default.
- W2139968664 created "2016-06-24" @default.
- W2139968664 creator A5011643819 @default.
- W2139968664 creator A5029988563 @default.
- W2139968664 creator A5055692722 @default.
- W2139968664 creator A5082654213 @default.
- W2139968664 creator A5089545879 @default.
- W2139968664 creator A5003841411 @default.
- W2139968664 date "1995-07-01" @default.
- W2139968664 modified "2023-09-24" @default.
- W2139968664 title "Oligodendroglial development in human fetal cerebrum" @default.
- W2139968664 cites W1480361759 @default.
- W2139968664 cites W1525813631 @default.
- W2139968664 cites W1526030258 @default.
- W2139968664 cites W1551700830 @default.
- W2139968664 cites W165672703 @default.
- W2139968664 cites W16585500 @default.
- W2139968664 cites W1658993979 @default.
- W2139968664 cites W1899554279 @default.
- W2139968664 cites W1972524334 @default.
- W2139968664 cites W1977994351 @default.
- W2139968664 cites W1977994578 @default.
- W2139968664 cites W1987388499 @default.
- W2139968664 cites W1987885030 @default.
- W2139968664 cites W1995646093 @default.
- W2139968664 cites W1996634693 @default.
- W2139968664 cites W1998225973 @default.
- W2139968664 cites W2001888569 @default.
- W2139968664 cites W2002760259 @default.
- W2139968664 cites W2005272267 @default.
- W2139968664 cites W2007434276 @default.
- W2139968664 cites W2020798622 @default.
- W2139968664 cites W2021755100 @default.
- W2139968664 cites W2027983444 @default.
- W2139968664 cites W2034258321 @default.
- W2139968664 cites W2041583712 @default.
- W2139968664 cites W2050601632 @default.
- W2139968664 cites W2055259320 @default.
- W2139968664 cites W2060413384 @default.
- W2139968664 cites W2077492083 @default.
- W2139968664 cites W2081560586 @default.
- W2139968664 cites W2091394377 @default.
- W2139968664 cites W2095219481 @default.
- W2139968664 cites W2150929583 @default.
- W2139968664 cites W2159425270 @default.
- W2139968664 cites W2165278466 @default.
- W2139968664 cites W2188067471 @default.
- W2139968664 cites W2320764162 @default.
- W2139968664 cites W29671035 @default.
- W2139968664 doi "https://doi.org/10.1002/ana.410380116" @default.
- W2139968664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7611731" @default.
- W2139968664 hasPublicationYear "1995" @default.
- W2139968664 type Work @default.
- W2139968664 sameAs 2139968664 @default.
- W2139968664 citedByCount "62" @default.
- W2139968664 countsByYear W21399686642013 @default.
- W2139968664 countsByYear W21399686642014 @default.
- W2139968664 countsByYear W21399686642015 @default.
- W2139968664 countsByYear W21399686642016 @default.
- W2139968664 countsByYear W21399686642017 @default.
- W2139968664 countsByYear W21399686642018 @default.
- W2139968664 countsByYear W21399686642019 @default.
- W2139968664 countsByYear W21399686642020 @default.
- W2139968664 countsByYear W21399686642021 @default.
- W2139968664 countsByYear W21399686642023 @default.
- W2139968664 crossrefType "journal-article" @default.
- W2139968664 hasAuthorship W2139968664A5003841411 @default.
- W2139968664 hasAuthorship W2139968664A5011643819 @default.
- W2139968664 hasAuthorship W2139968664A5029988563 @default.
- W2139968664 hasAuthorship W2139968664A5055692722 @default.
- W2139968664 hasAuthorship W2139968664A5082654213 @default.
- W2139968664 hasAuthorship W2139968664A5089545879 @default.
- W2139968664 hasConcept C124964019 @default.
- W2139968664 hasConcept C142724271 @default.
- W2139968664 hasConcept C1491633281 @default.
- W2139968664 hasConcept C169760540 @default.
- W2139968664 hasConcept C172680121 @default.
- W2139968664 hasConcept C203014093 @default.
- W2139968664 hasConcept C204232928 @default.
- W2139968664 hasConcept C2776985911 @default.
- W2139968664 hasConcept C2777670902 @default.
- W2139968664 hasConcept C2778609137 @default.
- W2139968664 hasConcept C2779234561 @default.
- W2139968664 hasConcept C2781017270 @default.
- W2139968664 hasConcept C529278444 @default.
- W2139968664 hasConcept C54355233 @default.
- W2139968664 hasConcept C55493867 @default.
- W2139968664 hasConcept C71924100 @default.
- W2139968664 hasConcept C86803240 @default.
- W2139968664 hasConcept C95444343 @default.
- W2139968664 hasConceptScore W2139968664C124964019 @default.
- W2139968664 hasConceptScore W2139968664C142724271 @default.
- W2139968664 hasConceptScore W2139968664C1491633281 @default.
- W2139968664 hasConceptScore W2139968664C169760540 @default.
- W2139968664 hasConceptScore W2139968664C172680121 @default.
- W2139968664 hasConceptScore W2139968664C203014093 @default.
- W2139968664 hasConceptScore W2139968664C204232928 @default.
- W2139968664 hasConceptScore W2139968664C2776985911 @default.