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- W2139968777 abstract "Research Article1 February 1985free access Three distinct epitopes within the loop region of hen egg lysozyme defined with monoclonal antibodies. M.J. Darsley M.J. Darsley Search for more papers by this author A.R. Rees A.R. Rees Search for more papers by this author M.J. Darsley M.J. Darsley Search for more papers by this author A.R. Rees A.R. Rees Search for more papers by this author Author Information M.J. Darsley and A.R. Rees The EMBO Journal (1985)4:383-392https://doi.org/10.1002/j.1460-2075.1985.tb03640.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Five monoclonal antibodies specific for the loop region of hen egg lysozyme were prepared by immunisation with a synthetic conjugate of a proteolytic fragment of lysozyme coupled to bovine serum albumin. Their fine specificities were investigated using a panel of variant lysozymes and peptide fragments of lysozyme in a quantitative radio-immunoassay procedure. Knowledge of the structure of hen lysozyme to high resolution and the use of computer graphics enables the localisation of the epitopes recognised by the antibodies with some precision. The antibodies were shown to define three distinct, overlapping epitopes within what was previously considered to be a single antigenic site. These results are discussed in relation to current ideas of the antigenic nature of proteins and other recent studies in which anti-protein antibodies have been elicited by immunisation with small peptides. Previous ArticleNext Article Volume 4Issue 21 February 1985In this issue RelatedDetailsLoading ..." @default.
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- W2139968777 title "Three distinct epitopes within the loop region of hen egg lysozyme defined with monoclonal antibodies." @default.
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- W2139968777 doi "https://doi.org/10.1002/j.1460-2075.1985.tb03640.x" @default.
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