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• W2139999335 abstract "Photo-synchronized pupae were used to determine the titer of ecdysteroids during the pupal and pharate adult stages of the Indianmeal moth, Plodia interpunctella (Hiibner). For female pupae that were kept in a 16h 1ight:S h dark (longday) photocycle, there was one major peak of ecdysteroids. The ecdysteroids reached a maximum of 2000-2500 pg/mg wet wt between 28-36 h after pupation. The ecdysteroids declined to 400 pglmg wet wt by 68 h after pupation. For pupae that were kept in continuous darkwss, the ecdysteroid peak became broader with a maximum at 24 h (3130 pg/mg wet wt). The composition of ecdysteroids at various times during pharate adult development was determined usiug gas chromatographass spectrometry with selected ion monitoring (GC-MS (S1M)I. Eedysone was the major component (93%) in samples taken 28 h after pupation, at the maximum of the pupal peak. Thus, high levels of ecdysoue are correlated with the beginning of adult ovarian development. As pharate development progressed, the proportion of 20-hydroxyecdysow increased so that by 100 h after pupation 20-hydroxyecdysone was the only ecdysteroid detected. Previously, the initiation of vitellogenesis was determined to begin between % and 100 h after pupation. In conjuction with previous work, these findings demonstrate that the ecdysteroid titers decline before viteUogenesis is initiated and egg maturation can be completed. Ecdysteroids Vitellogenesis Metamorphosis Radioimrnunoassay GC-MS quantification" @default.
• W2139999335 created "2016-06-24" @default.
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• W2139999335 date "1993-01-01" @default.
• W2139999335 modified "2023-10-06" @default.
• W2139999335 title "Declining ecdysteroid levels are temporally correlated with the initiation of vitellogenesis during pharate adult development in the Indianmeal moth, Plodia interpunctella" @default.
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• W2139999335 doi "https://doi.org/10.1016/0965-1748(93)90094-9" @default.
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