FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140000006> ?p ?o ?g. }

• W2140000006 abstract "The delivery of DNA into human cells has been the basis of advances in the understanding of gene function and the development of genetic therapies. Numerous chemical and physical approaches have been used to deliver the DNA, but their efficacy has been variable and is highly dependent on the cell type to be transfected.Studies were undertaken to evaluate and compare the transfection efficacy of several chemical reagents to that of the electroporation/nucleofection system using both adherent cells (primary and transformed airway epithelial cells and primary fibroblasts as well as embryonic stem cells) and cells in suspension (primary hematopoietic stem/progenitor cells and lymphoblasts). With the exception of HEK 293 cell transfection, nucleofection proved to be less toxic and more efficient at effectively delivering DNA into the cells as determined by cell proliferation and GFP expression, respectively. Lipofectamine and nucleofection of HEK 293 were essentially equivalent in terms of toxicity and efficiency. Transient transfection efficiency in all the cell systems ranged from 40%-90%, with minimal toxicity and no apparent species specificity. Differences in efficiency and toxicity were cell type/system specific.In general, the Amaxa electroporation/nucleofection system appears superior to other chemical systems. However, there are cell-type and species specific differences that need to be evaluated empirically to optimize the conditions for transfection efficiency and cell survival." @default.
• W2140000006 created "2016-06-24" @default.
• W2140000006 creator A5016355973 @default.
• W2140000006 creator A5023616396 @default.
• W2140000006 creator A5038471138 @default.
• W2140000006 creator A5046074910 @default.
• W2140000006 creator A5053789515 @default.
• W2140000006 creator A5075930347 @default.
• W2140000006 creator A5090124272 @default.
• W2140000006 date "2010-02-08" @default.
• W2140000006 modified "2023-10-10" @default.
• W2140000006 title "Comparative transfection of DNA into primary and transformed mammalian cells from different lineages" @default.
• W2140000006 cites W1492703324 @default.
• W2140000006 cites W1501353826 @default.
• W2140000006 cites W1579604458 @default.
• W2140000006 cites W1588243510 @default.
• W2140000006 cites W1967626549 @default.
• W2140000006 cites W1990453550 @default.
• W2140000006 cites W1993066368 @default.
• W2140000006 cites W1993993814 @default.
• W2140000006 cites W2001675896 @default.
• W2140000006 cites W2005644246 @default.
• W2140000006 cites W2009119435 @default.
• W2140000006 cites W2011340684 @default.
• W2140000006 cites W2019364665 @default.
• W2140000006 cites W2019502784 @default.
• W2140000006 cites W2028893284 @default.
• W2140000006 cites W2029812891 @default.
• W2140000006 cites W2039961929 @default.
• W2140000006 cites W2044296969 @default.
• W2140000006 cites W2044388752 @default.
• W2140000006 cites W2049996097 @default.
• W2140000006 cites W2056890189 @default.
• W2140000006 cites W2057846579 @default.
• W2140000006 cites W2065784422 @default.
• W2140000006 cites W2069068158 @default.
• W2140000006 cites W2070122091 @default.
• W2140000006 cites W2071496358 @default.
• W2140000006 cites W2080008193 @default.
• W2140000006 cites W2080989437 @default.
• W2140000006 cites W2086668227 @default.
• W2140000006 cites W2113894208 @default.
• W2140000006 cites W2117145783 @default.
• W2140000006 cites W2117539939 @default.
• W2140000006 cites W2148273027 @default.
• W2140000006 cites W2152307544 @default.
• W2140000006 cites W2158228334 @default.
• W2140000006 doi "https://doi.org/10.1186/1472-6750-10-9" @default.
• W2140000006 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2830169" @default.
• W2140000006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20144189" @default.
• W2140000006 hasPublicationYear "2010" @default.
• W2140000006 type Work @default.
• W2140000006 sameAs 2140000006 @default.
• W2140000006 citedByCount "139" @default.
• W2140000006 countsByYear W21400000062012 @default.
• W2140000006 countsByYear W21400000062013 @default.
• W2140000006 countsByYear W21400000062014 @default.
• W2140000006 countsByYear W21400000062015 @default.
• W2140000006 countsByYear W21400000062016 @default.
• W2140000006 countsByYear W21400000062017 @default.
• W2140000006 countsByYear W21400000062018 @default.
• W2140000006 countsByYear W21400000062019 @default.
• W2140000006 countsByYear W21400000062020 @default.
• W2140000006 countsByYear W21400000062021 @default.
• W2140000006 countsByYear W21400000062022 @default.
• W2140000006 countsByYear W21400000062023 @default.
• W2140000006 crossrefType "journal-article" @default.
• W2140000006 hasAuthorship W2140000006A5016355973 @default.
• W2140000006 hasAuthorship W2140000006A5023616396 @default.
• W2140000006 hasAuthorship W2140000006A5038471138 @default.
• W2140000006 hasAuthorship W2140000006A5046074910 @default.
• W2140000006 hasAuthorship W2140000006A5053789515 @default.
• W2140000006 hasAuthorship W2140000006A5075930347 @default.
• W2140000006 hasAuthorship W2140000006A5090124272 @default.
• W2140000006 hasBestOaLocation W21400000061 @default.
• W2140000006 hasConcept C104317684 @default.
• W2140000006 hasConcept C144251240 @default.
• W2140000006 hasConcept C145103041 @default.
• W2140000006 hasConcept C147905033 @default.
• W2140000006 hasConcept C153911025 @default.
• W2140000006 hasConcept C174510640 @default.
• W2140000006 hasConcept C186852380 @default.
• W2140000006 hasConcept C201750760 @default.
• W2140000006 hasConcept C28328180 @default.
• W2140000006 hasConcept C40767141 @default.
• W2140000006 hasConcept C49923114 @default.
• W2140000006 hasConcept C54009773 @default.
• W2140000006 hasConcept C54355233 @default.
• W2140000006 hasConcept C81885089 @default.
• W2140000006 hasConcept C86803240 @default.
• W2140000006 hasConcept C92087593 @default.
• W2140000006 hasConcept C95444343 @default.
• W2140000006 hasConceptScore W2140000006C104317684 @default.
• W2140000006 hasConceptScore W2140000006C144251240 @default.
• W2140000006 hasConceptScore W2140000006C145103041 @default.
• W2140000006 hasConceptScore W2140000006C147905033 @default.
• W2140000006 hasConceptScore W2140000006C153911025 @default.
• W2140000006 hasConceptScore W2140000006C174510640 @default.
• W2140000006 hasConceptScore W2140000006C186852380 @default.
• W2140000006 hasConceptScore W2140000006C201750760 @default.

• next