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• W2140030143 abstract "The atomic force microscope (AFM) can be used to visualize and to manipulate biological material with relative ease and high resolution. This study was carried out to investigate whether probe sets, specific for subregions of the human genome and useful for the painting of chromosome bands, can be established by PCR amplification of AFM-dissected chromosome regions. Compared to standard microdissection techniques, the AFM can be used with much higher precision for the dissection of the region of interest and subsequent nanoextraction of DNA material. After scanning the area of interest in noncontact mode AFM, chromosome bands were cut by the AFM tip at high force. The genetic material of a single cut attached itself to the tip and was extracted and amplified using degenerate oligonucleotide-primed–PCR. Subsequent to hapten labeling, fluorescencein situhybridization was performed and chromosome band-specific probes were visualized by standard fluorescence microscopy." @default.
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• W2140030143 date "1997-07-01" @default.
• W2140030143 modified "2023-10-16" @default.
• W2140030143 title "The Atomic Force Microscope as a New Microdissecting Tool for the Generation of Genetic Probes" @default.
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• W2140030143 doi "https://doi.org/10.1006/jsbi.1997.3869" @default.
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