FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140038668> ?p ?o ?g. }

• W2140038668 endingPage "213" @default.
• W2140038668 startingPage "207" @default.
• W2140038668 abstract "Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0.6 µm, 1 µm and 1.6 µm), DNA and microcarrier concentrations (0.5 µg of DNA with 150 µg of gold particles or 1.0 µg of DNA with 300 µg of gold particles/bombardment) and bombardment numbers (1x (single) and 2x (double)) were assessed. The effect of high osmoticum in the bombardment medium (0.3 M mannitol and 0.4 M maltose) and the age of embryos were also evaluated. Optimal expression in MDEs was obtained using the following conditions of double bombardment at 1350 psi, 9 cm target distance, a 1 µm gold particle size, 1.0 µg of DNA with 300 µg of gold particles/bombardment, and osmotic pretreatment of 4-6 weeks old embryos using 0.4 M maltose for 6 h before and 16 h after bombardment. The optimized transformation protocol presented in this study is expected to improve devalopment of commercial transgenic wheat lines expressing" @default.
• W2140038668 created "2016-06-24" @default.
• W2140038668 creator A5003699159 @default.
• W2140038668 creator A5014092258 @default.
• W2140038668 creator A5040970881 @default.
• W2140038668 creator A5066877248 @default.
• W2140038668 date "2008-10-01" @default.
• W2140038668 modified "2023-09-24" @default.
• W2140038668 title "Optimization of transient expression of uidA gene in androgenic embryos of wheat (Triticum aestivum L. cv. Falat) via particle bombardment" @default.
• W2140038668 cites W1255757723 @default.
• W2140038668 cites W1535619091 @default.
• W2140038668 cites W1594569806 @default.
• W2140038668 cites W1965170150 @default.
• W2140038668 cites W1976876678 @default.
• W2140038668 cites W1986587865 @default.
• W2140038668 cites W2003553561 @default.
• W2140038668 cites W2007723086 @default.
• W2140038668 cites W2015074203 @default.
• W2140038668 cites W2037665013 @default.
• W2140038668 cites W2039376711 @default.
• W2140038668 cites W2039806753 @default.
• W2140038668 cites W2041640817 @default.
• W2140038668 cites W2044946188 @default.
• W2140038668 cites W2048641070 @default.
• W2140038668 cites W2057120761 @default.
• W2140038668 cites W2058640003 @default.
• W2140038668 cites W2070179228 @default.
• W2140038668 cites W2070381753 @default.
• W2140038668 cites W2073156154 @default.
• W2140038668 cites W2074712170 @default.
• W2140038668 cites W2075943003 @default.
• W2140038668 cites W2081889616 @default.
• W2140038668 cites W2087532158 @default.
• W2140038668 cites W2104926457 @default.
• W2140038668 cites W2113348446 @default.
• W2140038668 cites W2119898083 @default.
• W2140038668 cites W2129156292 @default.
• W2140038668 cites W2131140715 @default.
• W2140038668 cites W2135369487 @default.
• W2140038668 cites W2148681645 @default.
• W2140038668 cites W2185942971 @default.
• W2140038668 cites W2323349288 @default.
• W2140038668 cites W2497889913 @default.
• W2140038668 cites W2784062903 @default.
• W2140038668 cites W326735233 @default.
• W2140038668 cites W89323328 @default.
• W2140038668 hasPublicationYear "2008" @default.
• W2140038668 type Work @default.
• W2140038668 sameAs 2140038668 @default.
• W2140038668 citedByCount "1" @default.
• W2140038668 crossrefType "journal-article" @default.
• W2140038668 hasAuthorship W2140038668A5003699159 @default.
• W2140038668 hasAuthorship W2140038668A5014092258 @default.
• W2140038668 hasAuthorship W2140038668A5040970881 @default.
• W2140038668 hasAuthorship W2140038668A5066877248 @default.
• W2140038668 hasConcept C104317684 @default.
• W2140038668 hasConcept C121807389 @default.
• W2140038668 hasConcept C12554922 @default.
• W2140038668 hasConcept C1491633281 @default.
• W2140038668 hasConcept C153911025 @default.
• W2140038668 hasConcept C176999471 @default.
• W2140038668 hasConcept C182420341 @default.
• W2140038668 hasConcept C185592680 @default.
• W2140038668 hasConcept C18903297 @default.
• W2140038668 hasConcept C196843134 @default.
• W2140038668 hasConcept C2778197599 @default.
• W2140038668 hasConcept C2778445172 @default.
• W2140038668 hasConcept C2778517922 @default.
• W2140038668 hasConcept C2778597767 @default.
• W2140038668 hasConcept C2780618852 @default.
• W2140038668 hasConcept C29172406 @default.
• W2140038668 hasConcept C54355233 @default.
• W2140038668 hasConcept C552990157 @default.
• W2140038668 hasConcept C55493867 @default.
• W2140038668 hasConcept C59822182 @default.
• W2140038668 hasConcept C86803240 @default.
• W2140038668 hasConceptScore W2140038668C104317684 @default.
• W2140038668 hasConceptScore W2140038668C121807389 @default.
• W2140038668 hasConceptScore W2140038668C12554922 @default.
• W2140038668 hasConceptScore W2140038668C1491633281 @default.
• W2140038668 hasConceptScore W2140038668C153911025 @default.
• W2140038668 hasConceptScore W2140038668C176999471 @default.
• W2140038668 hasConceptScore W2140038668C182420341 @default.
• W2140038668 hasConceptScore W2140038668C185592680 @default.
• W2140038668 hasConceptScore W2140038668C18903297 @default.
• W2140038668 hasConceptScore W2140038668C196843134 @default.
• W2140038668 hasConceptScore W2140038668C2778197599 @default.
• W2140038668 hasConceptScore W2140038668C2778445172 @default.
• W2140038668 hasConceptScore W2140038668C2778517922 @default.
• W2140038668 hasConceptScore W2140038668C2778597767 @default.
• W2140038668 hasConceptScore W2140038668C2780618852 @default.
• W2140038668 hasConceptScore W2140038668C29172406 @default.
• W2140038668 hasConceptScore W2140038668C54355233 @default.
• W2140038668 hasConceptScore W2140038668C552990157 @default.
• W2140038668 hasConceptScore W2140038668C55493867 @default.
• W2140038668 hasConceptScore W2140038668C59822182 @default.
• W2140038668 hasConceptScore W2140038668C86803240 @default.
• W2140038668 hasIssue "4" @default.

• next