Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140138547> ?p ?o ?g. }
- W2140138547 abstract "Protein biomarkers in bodily fluids can lead to an indication of the onset of a disease. Due to low abundance of the biomarker at early stages of the disease and other protein interferences, detection may be challenging. Therefore, in order to achieve more selective protein separations, the development of a novel stationary phase has been investigated. Moreover, novel methods to apply polysaccharides as stationary phases were developed for protein separations as well as chiral separations. The initial discussions in this dissertation commences with the use of novel zwitterionic lysine-based molecular micelles as a coating for open tubular capillary electrochromatography (OT-CEC) for protein separations. Poly-å-sodium-undecanoyl lysinate (poly-å-SUK) was evaluated as a stationary phase by varying polymer concentration, voltage, and temperature in the separation of six acidic (myoglobulin, albumin, â-lactoglobulin A, â-lactoglobulin B, á-lactalbumin, deoxyribonuclease I) and four basic (á-chymotrypsinogen A, lysozyme, cytochrome c, and ribonuclease A) proteins. Additionally, NaCl concentration was assessed for enhancement of the separation due to increased interactions from polymer swelling. Nearly all protein peaks were baseline resolved. In addition, a separation of proteins in a human serum sample was evaluated under optimized acidic and basic conditions. Furthermore, a separation of proteins in a human serum sample was also evaluated under optimized acidic and basic conditions. This dissertation also addresses chiral separations using polysaccharides derivatives (cellulose acetate (CA), cellulose acetate phthalate (CAP), and cellulose acetate butyrate (CAB)) for OT-CEC. These hydrophobic polymers were each dissolved in a room temperature ionic liquid (RTIL), 1-butyl-3-methylimidazolium acetate and deposited into capillaries. Four chiral analytes (thiopental, sotalol, labetalol, and ephedrine) were evaluated using the coated capillaries. The system was optimized using pH, polymer concentration, voltage, and temperature studies. Examination of results indicated baseline resolution for thiopental using each polysaccharide, while only partial separation was achieved for sotalol, labetalol, and ephedrine. Lastly, protein separations were evaluated using polysaccharides as coatings for OT-CEC. Basic proteins, mentioned earlier, were investigated using CA, CAP, and CAB. CA had the fastest analysis time, and it was further investigated in this study. Polymer concentration, temperature, and voltage studies were conducted to determine optimized conditions. In addition, proteins were evaluated using CA nanofibers. Nanofibers were coated at various times onto the capillary wall for the separation of basic proteins." @default.
- W2140138547 created "2016-06-24" @default.
- W2140138547 creator A5083251551 @default.
- W2140138547 date "2022-06-10" @default.
- W2140138547 modified "2023-10-18" @default.
- W2140138547 title "Development of innovative stationary phases for chiral and protein separations in open tubular capillary electrochromatography" @default.
- W2140138547 cites W1490627795 @default.
- W2140138547 cites W1576619377 @default.
- W2140138547 cites W1600292521 @default.
- W2140138547 cites W1963664510 @default.
- W2140138547 cites W1965538774 @default.
- W2140138547 cites W1965843819 @default.
- W2140138547 cites W1967865716 @default.
- W2140138547 cites W1970035147 @default.
- W2140138547 cites W1971427463 @default.
- W2140138547 cites W1971731968 @default.
- W2140138547 cites W1971790517 @default.
- W2140138547 cites W1972234603 @default.
- W2140138547 cites W1972291588 @default.
- W2140138547 cites W1972512054 @default.
- W2140138547 cites W1973036269 @default.
- W2140138547 cites W1975205743 @default.
- W2140138547 cites W1976069697 @default.
- W2140138547 cites W1976173373 @default.
- W2140138547 cites W1976569701 @default.
- W2140138547 cites W1979922698 @default.
- W2140138547 cites W1980573295 @default.
- W2140138547 cites W1980655040 @default.
- W2140138547 cites W1982676798 @default.
- W2140138547 cites W1983096011 @default.
- W2140138547 cites W1983496093 @default.
- W2140138547 cites W1983660649 @default.
- W2140138547 cites W1985381279 @default.
- W2140138547 cites W1986273581 @default.
- W2140138547 cites W1986313570 @default.
- W2140138547 cites W1987797774 @default.
- W2140138547 cites W1988081630 @default.
- W2140138547 cites W1988577039 @default.
- W2140138547 cites W1989367922 @default.
- W2140138547 cites W1989866262 @default.
- W2140138547 cites W1993501128 @default.
- W2140138547 cites W1993638045 @default.
- W2140138547 cites W1995386664 @default.
- W2140138547 cites W1995463658 @default.
- W2140138547 cites W1996584133 @default.
- W2140138547 cites W1999038572 @default.
- W2140138547 cites W1999173093 @default.
- W2140138547 cites W1999939425 @default.
- W2140138547 cites W2002365801 @default.
- W2140138547 cites W2004040714 @default.
- W2140138547 cites W2004304270 @default.
- W2140138547 cites W2006546468 @default.
- W2140138547 cites W2006585685 @default.
- W2140138547 cites W2007985864 @default.
- W2140138547 cites W2009002092 @default.
- W2140138547 cites W2009434206 @default.
- W2140138547 cites W2009576647 @default.
- W2140138547 cites W2010898983 @default.
- W2140138547 cites W2010963780 @default.
- W2140138547 cites W2012875979 @default.
- W2140138547 cites W2014496402 @default.
- W2140138547 cites W2015325558 @default.
- W2140138547 cites W2017863881 @default.
- W2140138547 cites W2019230621 @default.
- W2140138547 cites W2019863964 @default.
- W2140138547 cites W2021243063 @default.
- W2140138547 cites W2021800483 @default.
- W2140138547 cites W2022439150 @default.
- W2140138547 cites W2022731877 @default.
- W2140138547 cites W2022846918 @default.
- W2140138547 cites W2024105179 @default.
- W2140138547 cites W2024505283 @default.
- W2140138547 cites W2025213772 @default.
- W2140138547 cites W2025681873 @default.
- W2140138547 cites W2027466368 @default.
- W2140138547 cites W2027887549 @default.
- W2140138547 cites W2029101329 @default.
- W2140138547 cites W2029115860 @default.
- W2140138547 cites W2030278594 @default.
- W2140138547 cites W2031773818 @default.
- W2140138547 cites W2032735759 @default.
- W2140138547 cites W2033728551 @default.
- W2140138547 cites W2033920095 @default.
- W2140138547 cites W2034013153 @default.
- W2140138547 cites W2036718579 @default.
- W2140138547 cites W2037298831 @default.
- W2140138547 cites W2037433647 @default.
- W2140138547 cites W2038758161 @default.
- W2140138547 cites W2040117459 @default.
- W2140138547 cites W2045220325 @default.
- W2140138547 cites W2048047892 @default.
- W2140138547 cites W2050372426 @default.
- W2140138547 cites W2051646930 @default.
- W2140138547 cites W2053382886 @default.
- W2140138547 cites W2053533559 @default.
- W2140138547 cites W2054915174 @default.
- W2140138547 cites W2055901832 @default.
- W2140138547 cites W2056277164 @default.
- W2140138547 cites W2057395287 @default.
- W2140138547 cites W2058642113 @default.