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- W2140211769 abstract "Embryogenesis occurred in Nigella sativa L. (Fam. Ranunculaceae) leaf callus tissue when coconut milk was replaced from the Murashige and Skoog's (MS) medium by casein hydrolysate. On MS + IAA (0.5 mg/l) + casein hydrolysate (100 and 500 mg/l) medium, tissue gained a capacity of growing embryoids for a pro-longed culture period. At a concentration of 1000 mg/l casein hydrolysate suppressed the differentiating capacity after the fifth subculture. 2.4-D and kinetin had inhibitory effects on morphogenesis. Histology of the differentiated tissue revealed that the origin of roots, shoot buds and leaves were from groups of meristematic cells whereas embryoids were initiated by the repeated division of a single cell." @default.
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- W2140211769 date "1976-10-01" @default.
- W2140211769 modified "2023-10-16" @default.
- W2140211769 title "Embryogenesis and Differentiation in Nigella sativa Leaf Callus in vitro" @default.
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- W2140211769 doi "https://doi.org/10.1111/j.1399-3054.1976.tb04869.x" @default.
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