Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140259097> ?p ?o ?g. }
- W2140259097 endingPage "7790" @default.
- W2140259097 startingPage "7775" @default.
- W2140259097 abstract "Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present. To understand translational regulation in this system, we systematically mutated AUGs 1–4 and monitored polypeptide synthesis from plasmids and recombinant viruses. This identified sORF2 as a key regulatory element with opposing effects on PB1-F2 and PB1-N40 expression. We propose a model in which AUGs 1–4 are accessed by leaky ribosomal scanning, with sORF2 repressing synthesis of downstream PB1-F2. However, sORF2 also up-regulates PB1-N40 expression, most likely by a reinitiation mechanism that permits skipping of AUG4. Surprisingly, we also found that in contrast to plasmid-driven expression, viruses with improved AUG1 initiation contexts produced less PB1 in infected cells and replicated poorly, producing virions with elevated particle:PFU ratios. Analysis of the genome content of virus particles showed reduced packaging of the mutant segment 2 vRNAs. Overall, we conclude that segment 2 mRNA translation is regulated by a combination of leaky ribosomal scanning and reinitiation, and that the sequences surrounding the PB1 AUG codon are multifunctional, containing overlapping signals for translation initiation and for segment-specific packaging." @default.
- W2140259097 created "2016-06-24" @default.
- W2140259097 creator A5018562873 @default.
- W2140259097 creator A5022752899 @default.
- W2140259097 creator A5023445168 @default.
- W2140259097 creator A5025180662 @default.
- W2140259097 creator A5042123952 @default.
- W2140259097 creator A5048262248 @default.
- W2140259097 creator A5068581313 @default.
- W2140259097 creator A5075135611 @default.
- W2140259097 creator A5088084394 @default.
- W2140259097 date "2011-06-21" @default.
- W2140259097 modified "2023-09-25" @default.
- W2140259097 title "Overlapping signals for translational regulation and packaging of influenza A virus segment 2" @default.
- W2140259097 cites W1483301458 @default.
- W2140259097 cites W1525947002 @default.
- W2140259097 cites W1546570611 @default.
- W2140259097 cites W1827821010 @default.
- W2140259097 cites W1970697384 @default.
- W2140259097 cites W1971428926 @default.
- W2140259097 cites W1976798293 @default.
- W2140259097 cites W1988457839 @default.
- W2140259097 cites W1989605800 @default.
- W2140259097 cites W1990180184 @default.
- W2140259097 cites W1997910503 @default.
- W2140259097 cites W1997972646 @default.
- W2140259097 cites W2004027140 @default.
- W2140259097 cites W2008410413 @default.
- W2140259097 cites W2013593450 @default.
- W2140259097 cites W2014700582 @default.
- W2140259097 cites W2015359732 @default.
- W2140259097 cites W2030844589 @default.
- W2140259097 cites W2032051738 @default.
- W2140259097 cites W2040336090 @default.
- W2140259097 cites W2041311366 @default.
- W2140259097 cites W2043348992 @default.
- W2140259097 cites W2055697584 @default.
- W2140259097 cites W2060008923 @default.
- W2140259097 cites W2066903037 @default.
- W2140259097 cites W2072187285 @default.
- W2140259097 cites W2074202566 @default.
- W2140259097 cites W2081897504 @default.
- W2140259097 cites W2103904572 @default.
- W2140259097 cites W2105042928 @default.
- W2140259097 cites W2106174330 @default.
- W2140259097 cites W2107737193 @default.
- W2140259097 cites W2126169483 @default.
- W2140259097 cites W2128677433 @default.
- W2140259097 cites W2130251737 @default.
- W2140259097 cites W2133318493 @default.
- W2140259097 cites W2133897818 @default.
- W2140259097 cites W2133943387 @default.
- W2140259097 cites W2134489190 @default.
- W2140259097 cites W2135213915 @default.
- W2140259097 cites W2135519282 @default.
- W2140259097 cites W2137146240 @default.
- W2140259097 cites W2139359573 @default.
- W2140259097 cites W2151133415 @default.
- W2140259097 cites W2154128645 @default.
- W2140259097 cites W2155102822 @default.
- W2140259097 cites W2155236172 @default.
- W2140259097 cites W2157498427 @default.
- W2140259097 cites W2160488806 @default.
- W2140259097 cites W2160883150 @default.
- W2140259097 cites W2165774777 @default.
- W2140259097 cites W2167939067 @default.
- W2140259097 cites W2168339472 @default.
- W2140259097 cites W2168355693 @default.
- W2140259097 cites W2185492534 @default.
- W2140259097 cites W22632941 @default.
- W2140259097 cites W4252931792 @default.
- W2140259097 doi "https://doi.org/10.1093/nar/gkr487" @default.
- W2140259097 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3177217" @default.
- W2140259097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21693560" @default.
- W2140259097 hasPublicationYear "2011" @default.
- W2140259097 type Work @default.
- W2140259097 sameAs 2140259097 @default.
- W2140259097 citedByCount "59" @default.
- W2140259097 countsByYear W21402590972012 @default.
- W2140259097 countsByYear W21402590972013 @default.
- W2140259097 countsByYear W21402590972014 @default.
- W2140259097 countsByYear W21402590972015 @default.
- W2140259097 countsByYear W21402590972016 @default.
- W2140259097 countsByYear W21402590972017 @default.
- W2140259097 countsByYear W21402590972018 @default.
- W2140259097 countsByYear W21402590972019 @default.
- W2140259097 countsByYear W21402590972020 @default.
- W2140259097 countsByYear W21402590972021 @default.
- W2140259097 countsByYear W21402590972022 @default.
- W2140259097 countsByYear W21402590972023 @default.
- W2140259097 crossrefType "journal-article" @default.
- W2140259097 hasAuthorship W2140259097A5018562873 @default.
- W2140259097 hasAuthorship W2140259097A5022752899 @default.
- W2140259097 hasAuthorship W2140259097A5023445168 @default.
- W2140259097 hasAuthorship W2140259097A5025180662 @default.
- W2140259097 hasAuthorship W2140259097A5042123952 @default.
- W2140259097 hasAuthorship W2140259097A5048262248 @default.
- W2140259097 hasAuthorship W2140259097A5068581313 @default.