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- W2140262108 abstract "Ethane-utilizing Mycobacterium duvalii TA5 and Mycobacterium gilvum TA27 and propane-utilizing Mycobacterium chlorophenolicum TCE 28 isolated from soils were able to degrade methyl tert-butyl ether (MTBE) cometabolically with various organic acids and carbohydrates as a carbon source. Among these strains, M. duvalii TA5 showed the highest MTBE degradation activity. M. duvalii TA5 degraded 45% of 22.7 mmol MTBE L-1 in 7 d cometabolically with 500 mg L-1 of glucose. The maximal MTBE degradation rate (Vmax) and the half-saturation constant (Km) of MTBE by resting cells of M. duvalii TA5 were 1,030 nmol min-1 mg dry cells-1 and 6.8 mM, respectively. Among the various carbohydrates, glucose was the most effi cient carbon source for MTBE degradation by M. duvalii TA5. tert-Butyl formate (TBF), tert-butyl alcohol (TBA), 2-hydroxy isobutyric acid (HIBA), 2-propanol, acetone, and hydroxyacetone (HA) were identifi ed as metabolites of MTBE degradation by M. duvalii TA5. M. duvalii TA5 utilized both TBF and 2-propanol as sole energy and carbon sources. M. duvalii TA5 was able to degrade MTBE in the soil. The present study demonstrates that M. duvalii TA5 is eff ective in degrading MTBE and may be a useful tool for the bioremediation of MTBE-contaminated soil." @default.
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- W2140262108 date "2009-12-01" @default.
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- W2140262108 title "Biodegradation of Methyl tert-Butyl Ether by Mycobacterium spp." @default.
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