Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140286834> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2140286834 endingPage "527" @default.
- W2140286834 startingPage "521" @default.
- W2140286834 abstract "1. Thermolysin was acetylated by N-acetylimidazole for examination of the contribuition of tyrosyl residues to the enzymatic activity and the stability. In the acetylthermolysin with 43% of the caseinolytic activity, 17 tyrosyl residues and 7 lysyl residues were estimated to be acetylated. 2. Inactivation was completely restored by de-acetylation of O-acetyltyrosyl residues with 1 M hydroxylamine. Lineweaver-Burk plots of native and acetylated thermolysins showed that the inactivation of the acetylthermolysin was due to the lowering of the affinity for the substrate but not to the decrease of a maximum velocity. 3. The cooperative action of heating and the detergent diminished the extraordinary stability of thermolysin, whereas it was remarkably stable to heating and the detergent when they were acted alone. By acetylation thermolysin became instable even at room temperature in the presence of the detergent. 4. The important contribution of tyrosyl resideus to the enzymatic activity and to the remarkabel stability of thermolysin was discussed based on the results obtained so far." @default.
- W2140286834 created "2016-06-24" @default.
- W2140286834 creator A5031647910 @default.
- W2140286834 creator A5041808200 @default.
- W2140286834 creator A5091131545 @default.
- W2140286834 date "1972-09-01" @default.
- W2140286834 modified "2023-09-26" @default.
- W2140286834 title "The Change of Stability and Activity of Thermolysin by Acetylation with N-Acetylimidazole" @default.
- W2140286834 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a129931" @default.
- W2140286834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4634971" @default.
- W2140286834 hasPublicationYear "1972" @default.
- W2140286834 type Work @default.
- W2140286834 sameAs 2140286834 @default.
- W2140286834 citedByCount "11" @default.
- W2140286834 crossrefType "journal-article" @default.
- W2140286834 hasAuthorship W2140286834A5031647910 @default.
- W2140286834 hasAuthorship W2140286834A5041808200 @default.
- W2140286834 hasAuthorship W2140286834A5091131545 @default.
- W2140286834 hasConcept C104317684 @default.
- W2140286834 hasConcept C119157956 @default.
- W2140286834 hasConcept C181199279 @default.
- W2140286834 hasConcept C185020521 @default.
- W2140286834 hasConcept C185592680 @default.
- W2140286834 hasConcept C18903297 @default.
- W2140286834 hasConcept C2775999725 @default.
- W2140286834 hasConcept C2777289219 @default.
- W2140286834 hasConcept C2780340462 @default.
- W2140286834 hasConcept C55493867 @default.
- W2140286834 hasConcept C86803240 @default.
- W2140286834 hasConceptScore W2140286834C104317684 @default.
- W2140286834 hasConceptScore W2140286834C119157956 @default.
- W2140286834 hasConceptScore W2140286834C181199279 @default.
- W2140286834 hasConceptScore W2140286834C185020521 @default.
- W2140286834 hasConceptScore W2140286834C185592680 @default.
- W2140286834 hasConceptScore W2140286834C18903297 @default.
- W2140286834 hasConceptScore W2140286834C2775999725 @default.
- W2140286834 hasConceptScore W2140286834C2777289219 @default.
- W2140286834 hasConceptScore W2140286834C2780340462 @default.
- W2140286834 hasConceptScore W2140286834C55493867 @default.
- W2140286834 hasConceptScore W2140286834C86803240 @default.
- W2140286834 hasIssue "3" @default.
- W2140286834 hasLocation W21402868341 @default.
- W2140286834 hasLocation W21402868342 @default.
- W2140286834 hasOpenAccess W2140286834 @default.
- W2140286834 hasPrimaryLocation W21402868341 @default.
- W2140286834 hasRelatedWork W2025402148 @default.
- W2140286834 hasRelatedWork W2053825085 @default.
- W2140286834 hasRelatedWork W2074351572 @default.
- W2140286834 hasRelatedWork W2078595220 @default.
- W2140286834 hasRelatedWork W2090756039 @default.
- W2140286834 hasRelatedWork W2140286834 @default.
- W2140286834 hasRelatedWork W2237717580 @default.
- W2140286834 hasRelatedWork W2402820431 @default.
- W2140286834 hasRelatedWork W2880402987 @default.
- W2140286834 hasRelatedWork W4313491452 @default.
- W2140286834 hasVolume "72" @default.
- W2140286834 isParatext "false" @default.
- W2140286834 isRetracted "false" @default.
- W2140286834 magId "2140286834" @default.
- W2140286834 workType "article" @default.