FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2140304429> ?p ?o ?g. }

• W2140304429 endingPage "150" @default.
• W2140304429 startingPage "143" @default.
• W2140304429 abstract "The vertical growth stage is the most dangerous stage of melanoma and is often associated with a poor prognosis. The increased invasiveness and metastasis that is typical for vertically growing melanoma are mediated by the molecules of cell adhesion (particularly, integrins). Integrin αvβ3, which is abundantly expressed on melanoma cells with high metastatic potentials and is characterized by low expression levels in normal melanocytes, is potentially an attractive target for melanoma diagnostics and therapy. Integrin αvβ3 is known to recognize the arginine-glycine-aspartic (RGD) sequence, which has been found in a wide variety of its natural ligands. Here expression vectors bearing the genes of fusion proteins have been constructed for producing these proteins in Escherichia coli. Such fusion proteins consist of a peptidic 'address,' targeting the integrins on melanoma cells, linked to an 'adaptor' for the attachment of a diagnostic or toxic agent. The peptidic 'address' contains the RGD motif, which is stabilized by a disulfide bond to achieve the optimal receptor binding conformation. The 'adaptor' is a tetrameric protein, namely, streptavidin, that is able to achieve high-affinity binding of d-biotin (K(d) = 10(-15) M) and confer avidity to the address peptide. This binding ability facilitates the generation of anti-melanoma diagnostic and therapeutic agents using the appropriate biotin derivatives. These recombinant proteins were purified from the periplasm of E.coli using columns with 2-iminobiotin agarose and demonstrated an ability to adhere to the surface of murine and human melanoma cells." @default.
• W2140304429 created "2016-06-24" @default.
• W2140304429 creator A5018260853 @default.
• W2140304429 creator A5018759811 @default.
• W2140304429 creator A5039308675 @default.
• W2140304429 creator A5055523644 @default.
• W2140304429 creator A5073385630 @default.
• W2140304429 creator A5081072988 @default.
• W2140304429 date "2012-11-16" @default.
• W2140304429 modified "2023-09-27" @default.
• W2140304429 title "Preparation and functional evaluation of RGD-modified streptavidin targeting to integrin-expressing melanoma cells" @default.
• W2140304429 cites W124279715 @default.
• W2140304429 cites W126194730 @default.
• W2140304429 cites W1589837889 @default.
• W2140304429 cites W2000814339 @default.
• W2140304429 cites W2010121529 @default.
• W2140304429 cites W2020258942 @default.
• W2140304429 cites W2023319785 @default.
• W2140304429 cites W2040313839 @default.
• W2140304429 cites W2042274047 @default.
• W2140304429 cites W2046295572 @default.
• W2140304429 cites W2054619874 @default.
• W2140304429 cites W2055206234 @default.
• W2140304429 cites W2055294918 @default.
• W2140304429 cites W2074012988 @default.
• W2140304429 cites W2100837269 @default.
• W2140304429 cites W2102419539 @default.
• W2140304429 cites W2105300288 @default.
• W2140304429 cites W2115378502 @default.
• W2140304429 cites W2115900937 @default.
• W2140304429 cites W2136471260 @default.
• W2140304429 cites W2147000598 @default.
• W2140304429 cites W2163316860 @default.
• W2140304429 cites W2171394974 @default.
• W2140304429 doi "https://doi.org/10.1093/protein/gzs076" @default.
• W2140304429 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23161915" @default.
• W2140304429 hasPublicationYear "2012" @default.
• W2140304429 type Work @default.
• W2140304429 sameAs 2140304429 @default.
• W2140304429 citedByCount "4" @default.
• W2140304429 countsByYear W21403044292016 @default.
• W2140304429 countsByYear W21403044292018 @default.
• W2140304429 countsByYear W21403044292022 @default.
• W2140304429 crossrefType "journal-article" @default.
• W2140304429 hasAuthorship W2140304429A5018260853 @default.
• W2140304429 hasAuthorship W2140304429A5018759811 @default.
• W2140304429 hasAuthorship W2140304429A5039308675 @default.
• W2140304429 hasAuthorship W2140304429A5055523644 @default.
• W2140304429 hasAuthorship W2140304429A5073385630 @default.
• W2140304429 hasAuthorship W2140304429A5081072988 @default.
• W2140304429 hasBestOaLocation W21403044291 @default.
• W2140304429 hasConcept C104317684 @default.
• W2140304429 hasConcept C123894998 @default.
• W2140304429 hasConcept C1491633281 @default.
• W2140304429 hasConcept C153911025 @default.
• W2140304429 hasConcept C159654299 @default.
• W2140304429 hasConcept C170493617 @default.
• W2140304429 hasConcept C179223381 @default.
• W2140304429 hasConcept C185592680 @default.
• W2140304429 hasConcept C195687474 @default.
• W2140304429 hasConcept C203014093 @default.
• W2140304429 hasConcept C2776190903 @default.
• W2140304429 hasConcept C2777658100 @default.
• W2140304429 hasConcept C2778204606 @default.
• W2140304429 hasConcept C2779358239 @default.
• W2140304429 hasConcept C40767141 @default.
• W2140304429 hasConcept C502942594 @default.
• W2140304429 hasConcept C55493867 @default.
• W2140304429 hasConcept C85789140 @default.
• W2140304429 hasConcept C86803240 @default.
• W2140304429 hasConcept C95444343 @default.
• W2140304429 hasConceptScore W2140304429C104317684 @default.
• W2140304429 hasConceptScore W2140304429C123894998 @default.
• W2140304429 hasConceptScore W2140304429C1491633281 @default.
• W2140304429 hasConceptScore W2140304429C153911025 @default.
• W2140304429 hasConceptScore W2140304429C159654299 @default.
• W2140304429 hasConceptScore W2140304429C170493617 @default.
• W2140304429 hasConceptScore W2140304429C179223381 @default.
• W2140304429 hasConceptScore W2140304429C185592680 @default.
• W2140304429 hasConceptScore W2140304429C195687474 @default.
• W2140304429 hasConceptScore W2140304429C203014093 @default.
• W2140304429 hasConceptScore W2140304429C2776190903 @default.
• W2140304429 hasConceptScore W2140304429C2777658100 @default.
• W2140304429 hasConceptScore W2140304429C2778204606 @default.
• W2140304429 hasConceptScore W2140304429C2779358239 @default.
• W2140304429 hasConceptScore W2140304429C40767141 @default.
• W2140304429 hasConceptScore W2140304429C502942594 @default.
• W2140304429 hasConceptScore W2140304429C55493867 @default.
• W2140304429 hasConceptScore W2140304429C85789140 @default.
• W2140304429 hasConceptScore W2140304429C86803240 @default.
• W2140304429 hasConceptScore W2140304429C95444343 @default.
• W2140304429 hasIssue "2" @default.
• W2140304429 hasLocation W21403044291 @default.
• W2140304429 hasLocation W21403044292 @default.
• W2140304429 hasOpenAccess W2140304429 @default.
• W2140304429 hasPrimaryLocation W21403044291 @default.
• W2140304429 hasRelatedWork W1815017480 @default.
• W2140304429 hasRelatedWork W1964637580 @default.

• next