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• W2140306716 abstract "Abstract The removal of bottlenecks in discovery stage metabolite identification studies is an ongoing challenge for the pharmaceutical industry. We describe the use of an ‘All‐in‐One’ approach to metabolite characterization that leverages the fast scanning and high mass accuracy of hybrid quadrupole time‐of‐flight mass spectrometry (QqToFMS) instruments. Full‐scan MS and MS/MS data is acquired using collision energy switching without the preselection, either manually or in a data‐dependent manner, of precursor ions. The acquisition of ‘clean’ MS/MS data is assisted by the use of ultrahigh‐performance chromatography. Data acquired using this method can then be mined post‐acquisition in a number of ways. These include using narrow window extracted ion chromatograms (nwXICs) for expected biotransformations, XICs for the product ions of the parent compound and/or expected modification of these product ions, and neutral loss chromatograms. This approach has the potential to be truly comprehensive for the determination of in vitro biotransformations in a drug discovery environment. Copyright © 2005 John Wiley & Sons, Ltd." @default.
• W2140306716 created "2016-06-24" @default.
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• W2140306716 date "2005-08-16" @default.
• W2140306716 modified "2023-10-18" @default.
• W2140306716 title "‘All-in-One’ analysis for metabolite identification using liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry with collision energy switching" @default.
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• W2140306716 doi "https://doi.org/10.1002/rcm.2101" @default.
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